J Korean Ophthalmol Soc.
2006 Oct;47(10):1654-1661.
Expression of IRFs and iNOS in the Mouse Cornea with Pseudomonas Aeruginosa Keratitis
- Affiliations
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- 1Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea. jjhlee@plaza.snu.ac.kr
- 2Seoul Artificial Eye Center, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea.
- 3Department of Ophthalmology, Seoul National University Bundang Hospital, Sungnam, Korea.
- 4Department of Ophthalmology, School of Medicine, Johns Hopkins University, Baltimore, USA.
- 5Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, USA.
- 6Bascom Palmer Eye Institute, University of Miami, Florida, USA.
Abstract
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PURPOSE: To investigate the expression of IRF-1, IRF-7 and iNOS in the mice model of Pseudomonas aeruginosa keratitis. The enzymatic activity of iNOS and its expression were also investigated.
METHODS
With western blot analysis, the protein expression of IRF-1, IRF-7 (at 24 hours), and iNOS (at 12 hours and 24 hours) were evaluated in the mouse model of P. aeruginosa keratitis. iNOS enzymatic activity was determined with a scintillation counter. IRF-1 and IRF-7 expression were localized with immunofluorescent labeling. The wounded control group was given the same corneal wound without bacterial inoculation, and the fellow eyes served as normal controls.
RESULTS
Expression of IRF-1, IRF-7 and iNOS was highly upregulated in corneas with P. aeruginosa keratitis compared to normal or wounded corneas. iNOS enzymatic activity also was higher in infected than normal corneas. In wounded corneas, NOS2 expression and activity slightly increased at 12 hours after the infection. Intense IRF-1 immunopositivity was seen in the epithelial layer of infected corneas. Some corneal stromal cells and endothelial cells showed moderate positive labeling in infected corneas. IRF-7 showed intense labeling in the epithelial layer and endothelial cells of normal as well as infected corneas. Increased IRF-7 labeling was observed in epithelial cells in the ulcerated region of infected corneas.
CONCLUSIONS
These results suggest that IRF-1, IRF-7 and iNOS may play a regulatory role in the immune responses and wound healing process in P. aeruginosa keratitis.
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