J Korean Rheum Assoc.  2002 Sep;9(3):190-198.

Polymorphism in the Promoter of CD14 Gene in Patients with Systemic Lupus Erythematosus and Rheumatoid Arthritis

• Min JK
• Lee HJ
• Park SH
• Cho CS
• Kim HY

Abstract

OBJECTIVE
CD14 is a receptor for lipopolysaccharide and is found as a plasma-membrane- anchored molecule on the surface of monocytes, macrophages, and granulocytes as well as in a soluble serum protein (sCD14). Single nucleotide polymorphism at position -159 in the promoter of CD 14 has been found to be associated with levels of sCD14 and with total serum immunoglobulin E (IgE). Increased circulating soluble CD14 (sCD14) and IgE levels are observed in patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Therefore, we are intended to investigate whether -159C/T polymorphism in the promoter of CD14 are associated with SLE and RA.
METHODS
We performed a polymerase chain reaction of the promoter of CD14 gene on genomic DNA from 201 SLE patients, 175 RA patients, and 245 healthy controls. -159C/T polymorphisms in the promoter of CD14 were determined by restriction fragment-length polymorphism (RFLP). Serum levels of sCD14 were measured by enzyme-linked immunoabsorbent assay in patients with SLE.
RESULTS
There was no significant difference between the CD14 promoter genotypes of SLE patients and the controls. The polymorphism was not associated with the concentration of sCD14 or serum IgE in patients with SLE. In contrast, RA patients had a higher frequency of the T allele (63.7 % vs 56.5 %, odds ratio 0.741, 95% confidence interval 0.553-0.991, p=0.043) and TT homozygote (37.7 % vs 27.0 %, odds ratio 1.642, 95% confidence interval 1.060-2.544, p=0.025) than the control.
CONCLUSION
Our results suggest that the CD14 promoter polymorphism (C -159T) might be one of the genetic risk factors for RA in Korean.

Keyword

CD14, Polymorphism; Systemic lupus erythematosus; Rheumatoid arthritis

MeSH Terms

Alleles
Arthritis, Rheumatoid*
DNA
Genotype
Granulocytes
Homozygote
Humans
Immunoglobulin E
Immunoglobulins
Lupus Erythematosus, Systemic*
Macrophages
Monocytes
Odds Ratio
Polymerase Chain Reaction
Polymorphism, Single Nucleotide
Risk Factors
DNA
Immunoglobulin E
Immunoglobulins