J Korean Soc Emerg Med.  2011 Jun;22(3):231-241.

Decrease of Proapolipoprotein AI was Associated with Poor Prognosis in Patients with Septic Shock

• Kwon WY
• Suh GJ
• Jo YH
• Kim K

Abstract

PURPOSE
Proteomics is a peptide screening approach to identify proteins related to physiological and pathological changes. Our objective was to analyze changes in serum proteome profiles in patients suffering from septic shock, during the first 24-h period of therapy and to determine whether these changes were associated with prognosis.
METHODS
This was a retrospective observational study based on prospectively collected data that was conducted in an emergency intensive care unit (ICU) of a tertiary referral hospital. Consecutive patients admitted to the ICU with septic shock were enrolled. We obtained serum samples from the patients at admission (0 h) and 24 h after admission (24 h). Then, we analyzed the serum proteome profiles, performed Western blots, and measured serum lipid profiles.
RESULTS
Twenty-seven patients were enrolled. Thirteen patients were grouped into the survivors (SURV) group and fourteen into the non-survivors group on day 30 (NON-SURV). Proteomic analyses and Western blot showed that the expression intensities of proapolipoprotein AI remained unchanged in SURV but decreased in NON-SURV during the first 24-h period of septic shock (p=0.015). Serum high density lipoprotein (HDL) cholesterol level also remained unchanged in SURV but decreased in NON-SURV (p=0.036).
CONCLUSION
The decrease in serum proapolipoprotein AI expression during the first 24-h period of therapy was associated with a consistently low serum HDL cholesterol level and a poor prognosis in patients with septic shock.

Keyword

Proteomics; Proapolipoprotein AI; High density lipoproteins; Prognosis; Shock; Septic

MeSH Terms

Apolipoprotein A-I
Blotting, Western
Cholesterol
Cholesterol, HDL
Emergencies
Humans
Intensive Care Units
Lipoproteins
Lipoproteins, HDL
Mass Screening
Prognosis
Prospective Studies
Proteins
Proteome
Proteomics
Retrospective Studies
Shock
Shock, Septic
Stress, Psychological
Survivors
Tertiary Care Centers
Apolipoprotein A-I
Cholesterol
Cholesterol, HDL
Lipoproteins
Lipoproteins, HDL
Proteins
Proteome