J Korean Continence Soc.  2001 Jun;5(1):39-47. 10.5213/jkcs.2001.5.1.39.

Serotonin(5-HT): can it be a cause in overactive bladder

Affiliations
  • 1Department of Urology, Dankook University, College of Medicine, Cheonan, Korea. shimwon2@anseo.dankook.ac.kr

Abstract

PURPOSE
The purpose of this study is to investigate in vitro the effects of serotonin on the rat detrusor. In particular, this study examines what drugs inhibit the serotonin-induced detrusor contractions.
MATERIALS AND METHODS
Isometric tension changes of isolated rat bladder muscle strips were recorded in an organ bath using a force transducer. Acute effects of serotonin (0.0001-0.01mM) were assessed on resting tension. Electrical field stimulation (EFS), bethanechol (0.0001-0.01mM), ATP (1-3mM) or KCl(63.5-254mM)-induced contractions using application in organ bath were compared with serotonin-induced contractions. In order to examine the action mechanism of serotonin-induced stimulation, EFS, bethanechol, ATP or KCl-induced contraction under serotonin (0.001mM) was assessed and serotonin (0.001 to 0.1mM) was cumulatively added to the organ bath following pre-incubation with propranolol, ketanserine, tropisetron, propiverine, sodium nitroprusside or doxazocin.
RESULTS
There are two phases to the serotonin-induced responsean initial transient contraction and a prolonged tonic phase. Serotonin produced a reversible and dose-dependent contraction of the detrusor strips. Responses to bethanechol significantly increased with a concentration of 0.001mM serotonin (p<0.05). There was no effect on the responses to ATP, KCl, or EFS under 0.001mM serotonin. The 5-HT2 receptor is mainly responsible for serotonin-induced contractions of the detrusor (p<0.05), while the 5-HT1 receptor is partially responsible. Doxazocin and propiverine each significantly suppressed the responses to serotonin, while sodium nitroprusside and tropisetron each had no effect (p<0.05).
CONCLUSIONS
Because the 5-HT2 antagonist blocked the effect of serotonin-induced bladder contractions and the stimulation of the adrenoreceptors, the 5-HT2 antagonist seems to improve lower urinary tract symptoms.

Keyword

Serotonin (5-HT); contractility; bladder

MeSH Terms

Adenosine Triphosphate
Animals
Baths
Bethanechol
Ketanserin
Lower Urinary Tract Symptoms
Nitroprusside
Propranolol
Rats
Receptors, Serotonin, 5-HT1
Serotonin
Serotonin 5-HT2 Receptor Antagonists
Transducers
Urinary Bladder
Urinary Bladder, Overactive*
Adenosine Triphosphate
Bethanechol
Ketanserin
Nitroprusside
Propranolol
Receptors, Serotonin, 5-HT1
Serotonin
Serotonin 5-HT2 Receptor Antagonists
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