Obstet Gynecol Sci.  2016 Jul;59(4):307-310. 10.5468/ogs.2016.59.4.307.

Embryonic development after exposure of mouse oocyte to various amount of ovarian endometriotic fluid

Affiliations
  • 1Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul, Korea.
  • 2Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. blasto@snubh.org
  • 3Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.

Abstract

This study assesses the fertilization and blastocyst-forming rate in mice cumulus-oocyte complexes (COCs) after the exposure of human ovarian endometriotic fluid. Endometriotic fluid was obtained from a single patient by aspiration at the time of a laparoscopic cystectomy and serially diluted. COCs were obtained from 46-week-old female BDF1 mice. After exposure to ovarian endometriotic fluid for five minutes, the COCs were washed three times and the oocytes were then fertilized by mice sperm. The fertilization and blastocyst formation rate and the proportion of hatching/hatched blastocyst in the four treatment groups were not inferior to those in non-exposure group.

Keyword

Blastocyst; Endometriotic fluid; Fertilization in vitro; Oocytes

MeSH Terms

Animals
Blastocyst
Cystectomy
Embryonic Development*
Female
Fertilization
Fertilization in Vitro
Humans
Mice*
Oocytes*
Pregnancy
Spermatozoa

Reference

1. Benaglia L, Bermejo A, Somigliana E, Scarduelli C, Ragni G, Fedele L, et al. Pregnancy outcome in women with endometriomas achieving pregnancy through IVF. Hum Reprod. 2012; 27:1663–1667.
2. Benaglia L, Cardellicchio L, Guarneri C, Paffoni A, Restelli L, Somigliana E, et al. IVF outcome in women with accidental contamination of follicular fluid with endometrioma content. Eur J Obstet Gynecol Reprod Biol. 2014; 181:130–134.
3. Suwajanakorn S, Pruksananonda K, Sereepapong W, Ahnonkitpanit V, Chompurat D, Boonkasemsanti W, et al. Effects of contaminated endometriotic contents on quality of oocytes. J Med Assoc Thai. 2001; 84:Suppl 1. S371–S376.
4. Khamsi F, Yavas Y, Lacanna IC, Roberge S, Endman M, Wong JC. Exposure of human oocytes to endometrioma fluid does not alter fertilization or early embryo development. J Assist Reprod Genet. 2001; 18:106–109.
5. Piromlertamorn W, Saeng-anan U, Vutyavanich T. Effects of ovarian endometriotic fluid exposure on fertilization rate of mouse oocytes and subsequent embryo development. Reprod Biol Endocrinol. 2013; 11:4.
6. Somigliana E, Infantino M, Benedetti F, Arnoldi M, Calanna G, Ragni G. The presence of ovarian endometriomas is associated with a reduced responsiveness to gonadotropins. Fertil Steril. 2006; 86:192–196.
7. Garcia-Velasco JA, Somigliana E. Management of endometriomas in women requiring IVF: to touch or not to touch. Hum Reprod. 2009; 24:496–501.
8. Ruiz-Flores FJ, Garcia-Velasco JA. Is there a benefit for surgery in endometrioma-associated infertility? Curr Opin Obstet Gynecol. 2012; 24:136–140.
9. Keyhan S, Hughes C, Price T, Muasher S. An update on surgical versus expectant management of ovarian endometriomas in infertile women. Biomed Res Int. 2015; 2015:204792.
Full Text Links
  • OGS
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr