Clin Exp Reprod Med.  2014 Mar;41(1):21-28. 10.5653/cerm.2014.41.1.21.

Relationship between follicular fluid adipocytokines and the quality of the oocyte and corresponding embryo development from a single dominant follicle in in vitro fertilization/intracytoplasmic sperm injection cycles

• Chang HJ
• Lee JH
• Lee JR
• Jee BC
• Suh CS
• Kim SH

• KMID: 2167486
• DOI: http://doi.org/10.5653/cerm.2014.41.1.21

Abstract

OBJECTIVE
To investigate the association of individual follicular fluid (FF) leptin and adiponectin levels with the quality of the corresponding oocyte and embryo.
METHODS
We prospectively enrolled 67 women who underwent controlled ovarian hyperstimulation with 89 FF samples. FF and the corresponding oocyte was obtained from a single dominant preovulatory follicle at the time of oocyte retrieval. Concentrations of leptin and adiponectin were measured by enzyme-linked immunosorbent assay in an individual follicle. The oocyte quality, fertilization rate, and corresponding embryo development were assessed.
RESULTS
The FF level of leptin was significantly associated with body mass index (r=0.334, p<0.01). The FF adiponectin level was significantly higher in the normal fertilization group than the abnormal fertilization group (p=0.009) in the non-obese women. A lower FF leptin level was associated with a trend toward mature oocytes, normal fertilization, and good embryo quality, although these relationships were not statistically significant. The leptin:adiponectin ratio of FF did not differ significantly according to oocyte and embryo quality. The quality of the oocyte and embryo was not associated with the FF leptin level tertile. However, the normal fertilization rate was positively associated with FF adiponectin level tertile. There was a trend towards improved oocytes and normal fertilization rates with the lowest tertile of the FF leptin:adiponectin ratio, but this difference was not statistically significant.
CONCLUSION
Our results suggest that a high FF adiponectin concentration could be a predictor of normal fertilization. However, the FF leptin concentration and leptin:adiponectin ratio is not significantly related to oocyte maturity and corresponding embryo development.

Keyword

Follicular fluid; Leptin; Adiponectin; Oocyte; Fertilization; Embryo

MeSH Terms

Adipokines*
Adiponectin
Body Mass Index
Embryonic Development*
Embryonic Structures*
Enzyme-Linked Immunosorbent Assay
Female
Fertilization
Follicular Fluid*
Humans
Leptin
Oocyte Retrieval
Oocytes*
Pregnancy
Prospective Studies
Spermatozoa*
Adipokines
Adiponectin
Leptin