Nat Prod Sci.  2016 Jun;22(2):77-81. 10.20307/nps.2016.22.2.77.

Simultaneous HPLC Analysis of Three Flavonoids in the Extracts of Artocarpus heterophyllus Heartwoods

Affiliations
  • 1Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand. pharkphoom.p@psu.ac.th
  • 2Excellent Research Laboratory, Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.

Abstract

A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of three antibacterial flavonoids, artocarpanone, artocarpin, and cycloartocarpin in ethyl acetate extracts from Artocarpus heterophyllus heartwoods. Separation was achieved using a TSK-gel ODS-80Tm column (5 µm, 4.6 × 150 mm) at 25℃ with a gradient elution system of methanol and water as follows: 0-8 min, 60:40; 8-27 min, 80:20; 27-35 min, 60:40, v/v, at a flow rate of 1 mL/min, and a quantitative UV detection at 285 nm. The method was validated by measuring the key parameters, including specificity, linearity, sensitivity, accuracy, repeatability and reproducibility. A high degree of specificity and sensitivity was achieved. The calibration curves for all three flavonoids showed good linearity with a coefficient of determinations (R²) of ≥ 0.9995. The recoveries of the method were from 98-104%, with good reproducibility and repeatability (RSD values of less than 2%) were also achieved. Ethyl acetate was the best solvent for extraction of these three flavonoids using the heat reflux conditions for 1 h. This optimized sample preparation and HPLC method can be practically used for a routine standardization process of the extracts from the A. heterophyllus heartwoods.

Keyword

Artocarpus heterophyllus; Artocarpanone; Artocarpin; Cycloartocarpin; HPLC

MeSH Terms

Artocarpus*
Calibration
Chromatography, High Pressure Liquid*
Flavonoids*
Hot Temperature
Methanol
Methods
Sensitivity and Specificity
Water
Flavonoids
Methanol
Water

Figure

  • Fig. 1. Chemical structures of artocarpanone (1), artocarpin (2), and cycloartocarpin (3).

  • Fig. 2. HPLC chromatograms of (A) authentic compounds an (B) heartwood extract of A. heterophyllus. (1: Artocarpanone, 2 Artocarpin, 3: Cycloartocarpin).

  • Fig. 3. HPLC chromatograms of hexane, chloroform, ethyl acetate, and methanol extracts from of A. heterophyllus heartwoods.


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