J Korean Soc Transplant.
2003 Dec;17(2):126-130.
Regeneration of Renal Tissue Using Cell Transplantation in a Renal Failure Rat Model
- Affiliations
-
- 1Department of Chemical Engineering, Hanyang University, Seoul, Korea. bskim@hanyang.ac.kr
- 2Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul, Korea.
- 3Department of Urology, Kangbuk Samsung Hospital, School of Medicine, Sungkyunkwan University, Seoul, Korea.
- 4Department of Pathology, Samsung Medical Center, School of Medicine, Sungkyunkwan University, Seoul, Korea.
- 5Department of Pathology, College of Medicine, Hanyang University, Seoul, Korea.
Abstract
- PURPOSE
Dialysis and renal transplantation, the current therapies for end-stage renal disease (ESRD), have limitations including severe complications, donor organ shortage, and graft failure. The present study investigated the possibility of using a tissue engineering technique for renal tissue reconstruction as a new method to replace the current treatments for ESRD. We restored renal structure in vivo by transplanting isolated renal cells in renal failure animal models.
METHODS
Renal failure was surgically induced by 5/6 nephrectomy using silk tie method in Sprague-Dawley (SD) rats. Renal failure was confirmed by measuring the concentrations of blood urea nitrogen (BUN) and creatinine from blood samples. Renal cells were freshly isolated from newborn SD rat kidneys and implanted into renal failure- induced kidneys with fibrin gel matrix for 4 weeks. Retrieved specimens were examined by histological analyses.
RESULTS
Renal failure-induced rats exhibited higher concentrations of BUN and creatinine compared to those of normal rats. Four weeks after cell transplantation, histological examination showed the reconstitution of vascular tufts of glomerular structures.
CONCLUSION
Renal failure rat models were successfully created by 5/6 nephrectomy. This study showed a possibility of restoring the renal structures by transplanting renal cells with fibrin gel matrix in renal failure rat models. Further studies, such as investigation on renal function recovery by cell transplantation, are necessary to determine the clinical utility of this method for partial or full replacement of renal structure and function in the treatment of ESRD.
MeSH Terms
-
Animals
Blood Urea Nitrogen
Cell Transplantation*
Creatinine
Dialysis
Fibrin
Humans
Infant, Newborn
Kidney
Kidney Failure, Chronic
Kidney Transplantation
Models, Animal*
Nephrectomy
Rats*
Rats, Sprague-Dawley
Recovery of Function
Regeneration*
Renal Insufficiency*
Silk
Tissue Donors
Tissue Engineering
Transplants*
Creatinine
Fibrin
Silk
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