Equivalent Lung Preservation of Newly Developed Low Potassium Solution with Euro-Collins Solution: Development of new lung preservation solution 1

Kim, Young Tae; Sung, Sook Whan; Kim, Joo Hyun

Abstract

Ischemia reperfusion injury is a major contributing cause to early graft failure in lung transplantation. To develop a new and more improved preservation solution for lung transplantation, we formulated basic compositions of new solution made by adding several additives to extracellular type dextran solution. The aim of this study was to compare the preservation effect of this newly composed solution (group 2) to that of the Euro-Collins solution (group 1) on postischemic lung function in a canine left lung transplantation model. Six adult mongrel dogs were used as subjects for left lung allotransplantation. Three donor dogs were flushed with the new solution (Group 2, n=3), while the remaining three were flushed with the Euro-Collins solution, serving as the control (Group 1, n=3). The allografts were stored in each preservation solution at 4oC. Left single lung transplantations were performed. After left lung allotransplamtation, lung functions were assessed at 10, 30, 60, and 120 min after reperfusion. Although better arterial oxygen tensions were observed in group 2, they were not statistically significant. The mean pulmonary arterial pressure showed no difference between two groups. Even though pulmonary vascular resistances were slightly higher in group 2, their differences showed no statistical significance. Autopsy findings revealed findings of pulmonary venous occlusion by thrombi and bronchial obstruction by granulation tissue in two cases of group 2. But it seemed that those findings were due to surgical failures or rejections rather than primary graft failures resulting from poor preservation. Compared to the Euro-Collins solution, the new solution demonstrated comparative allograft preservation in canine single left lung transplantation models. We concluded that this new solution could be used as a basic composition for the future development of ideal preservation solution.