Comparison of the Low Potassium Dextran Solution and ET-Kyoto Solution in Rabbit Lung

Lim, Cheong; Kim, Kyung Hwan; Kim, Young Tae; Sung, Sook Whan; Kim, Joo Hyun

Abstract

For improvement of lung preservation, many types of preservation solution were developed and tested. The aim of this study was to compare the effect of the most frequently used extracellular type preservation solution (Low Potassium Dextran, LPD) with a newly developed trehalose containing extracellular type preservation solution(ET-Kyoto, ETK) on postischemic lung function. Twelve New-Zealand white rabbit lungs were harvested and studied on an isolated, blood-perfused model of lung function after 4 hours of cold ischemia at 10degrees C. In group I (n=6), lungs were preserved with 100 mL/kg of LPD solution; in group II(n=6), lungs were preserved with 100 mL/kg of ETK solution. A few minutes before flushing with preservation solutions, 20micro gram of PGE1 were injected into main pulmonary artery. Functions of the preserved lung were compared with PO2, PA pressure, tracheal air pressure, and dry/wet ratio. The pulmonary efferent blood oxygen tension at the end of the 60-minute reperfusion period was higher in group II compared with group I(486.5+/-80.3 mmHg versus 432.5+/-82.9 mmHg at FiO2 1.0, p-value = NS). The mean pulmonary arterial pressure was similar in both groups.(33.7+/-2.2 mmHg versus 35.5+/-2.0 mmHg, p-value = NS). The peak inspiratory airway pressure was significantly lower in group II(8.0+/-0.6 mmHg versus 11.8+/-1.4 mmHg, p-value=0.02) The water content of the lung was lower in group II (70.2+/-6.9% versus 78.5+/-6.1%), but not significant. These data demonstrate that a newly-developed trehalose-containing ET-Kyoto solution yield equal or slightly superior lung function after reperfusion compared with LPD solution.