Tuberc Respir Dis.
2008 Dec;65(6):464-470.
Presumptive Role of Neutrophilic Oxidative Stress in Oxygen-induced Acute Lung Injury in Rats
- Affiliations
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- 1Department of Anatomy, Catholic University of Daegu, School of Medicine, Daegu, Korea.
- 2Department of Physiology, Catholic University of Daegu, School of Medicine, Daegu, Korea. leeym@cu.ac.kr
Abstract
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BACKGROUND: This study examined the role of neutrophilc oxidative stress in an O2-induced acute lung injury (ALI).
METHODS
For 48 h, experimental rats were exposed to pure oxygen (normobaric hyperoxia) in a plastic cage. Forty-eight hours after O2 breathing, the rats were sacrificed and the parameters for ALI associated with neutrophilic oxidative stress were assessed.
RESULTS
Normobaric pure oxygen induced ALI, which was quite similar to ARDS. The O2-induced neutrophilic oxidative stress was identified by confirming of the increase in lung myeloperoxidase, BAL neutrophils, malondialdehyde (MDA), cytosolic phospholipase A2 (cPLA2) activity in the lung, histological changes and BAL cytospin morphology.
CONCLUSION
In part, ALI-caused by oxygen is affected by neutrophils especially by the generation of free radicals.
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