Tuberc Respir Dis.  2006 Jul;61(1):54-59.

Usefulness of Automated PCR Test for the Detection of Mycobacterium Tuberculosis in Fresh Biopsy Tissues

• Choi WS
• Shin SY
• Kim JO
• Kim MS
• Lee HK

Abstract

BACKGROUND: Although there have been several studies regarding the clinical value of an automated TB-PCR study using sputum, bronchial washing, and other body fluid samples for the detection of pulmonary tuberculosis, there are only a few reports on the use of fresh tissue samples.
MATERIALS AND METHODS
The acid-fast bacilli stain(AFB), tuberculosis culture, automated TB-PCR study, and histopathology examination were performed in 42 fresh tissue samples.
RESULTS
Among the 42 cases, 18 cases were diagnosed with tuberculosis based on the clinical findings. Sixteen of the 18 cases were TB-PCR positive and of these 16 cases, only 2 cases were positive in the AFB stain or culture study. However, all 18 cases showed the histopathology findings of chronic granulomatous inflammation that was compatible with tuberculosis. Based on the clinical findings, the sensitivity, specificity, positive predictability, and negative predictability of the automated TB-PCR study were 88.9%, 100%, 100%, and 92.3% respectively.
CONCLUSION
An automated TB-PCR assay is an important diagnostic tool for diagnosing tuberculosis in fresh tissue samples.

Keyword

Fresh tissue sample; Automated tuberculosis polymerase chain reaction(TB-PCR)

MeSH Terms

Biopsy*
Body Fluids
Inflammation
Mycobacterium tuberculosis*
Mycobacterium*
Polymerase Chain Reaction*
Sputum
Tuberculosis
Tuberculosis, Pulmonary

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