Restor Dent Endod.  2014 Feb;39(1):24-31.

Micro-CT evaluation of internal adaptation in resin fillings with different dentin adhesives

• Han SH
• Park SH

Abstract

OBJECTIVES
The purpose of present study was to evaluate the internal adaptation of composite restorations using different adhesive systems.
MATERIALS AND METHODS
Typical class I cavities were prepared in 32 human third molars. The teeth were divided into the following four groups: 3-step etch-and-rinse, 2-step etch-and-rinse, 2-step self-etch and 1-step self-etch system were used. After the dentin adhesives were applied, composite resins were filled and light-cured in two layers. Then, silver nitrate solution was infiltrated, and all of the samples were scanned by micro-CT before and after thermo-mechanical load cycling. For each image, the length to which silver nitrate infiltrated, as a percentage of the whole pulpal floor length, was calculated (%SP). To evaluate the internal adaptation using conventional method, the samples were cut into 3 pieces by two sectioning at an interval of 1 mm in the middle of the cavity and they were dyed with Rhodamine-B. The cross sections of the specimens were examined by stereomicroscope. The lengths of the parts where actual leakage was shown were measured and calculated as a percentage of real leakage (%RP). The values for %SP and %RP were compared.
RESULTS
After thermo-mechanical loading, all specimens showed significantly increased %SP compared to before thermo-mechanical loading and 1-step self-etch system had the highest %SP (p < 0.05). There was a tendency for %SP and %RP to show similar microleakage percentage depending on its sectioning.
CONCLUSIONS
After thermo-mechanical load cycling, there were differences in internal adaptation among the groups using different adhesive systems.

Keyword

Dentin adhesive; Internal adaptation; Micro-CT; Microleakage

MeSH Terms

Adhesives*
Composite Resins
Dentin*
Humans
Methods
Molar, Third
Silver Nitrate
Tooth
Adhesives
Composite Resins
Silver Nitrate

Figure

• 

  Figure 1 Coronal (a) and sagittal (b) sections of micro-CT image. (a) In coronal section, 'v' marks indicate silver nitrate penetrated into microgap between tooth and restoration. '*' mark indicates silver nitrate which infiltrated into dentinal tubules; (b) The percentage of the silver nitrate penetration length into the microgap between the tooth and restoration with regard to the entire length of the pulpal floor (white dotted line) was calculated for each micro CT image (% SP). R, Resin; D, Dentin; P, Pulp.

• 

  Figure 2 Sagittal section of micro-CT image. (a) before thermo-mechanical loading; (b) after thermo-mechanical loading. 'v' marks indicate that silver nitrate penetrated into microgap between tooth and restoration. R, Resin; D, Dentin; P, Pulp.

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