Pediatr Allergy Respir Dis.
2008 Mar;18(1):37-45.
Evaluating the Allergenicity of Soybean by the Fermentation
- Affiliations
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- 1Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
- 2Department of Pediatrics, Dong-A University College of Medicine, Busan, Korea.
- 3Division of Applied Life Science, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea. ryu@gnu.ac.kr
Abstract
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PURPOSE: The aim of this study was to determine the allergenicity of soybean by fermentation.
METHODS
Non-fermented soybean, fermented soybean by Bacillus subtilis KFCC 11293 and 3-step fermented soybean by Lactococcus lactis subsp. lactis IFO 12007 and Aspergillus oryzae and B. subtilis KFCC 11293 were extracted with phosphate buffered saline (PBS). In order to detect soybean-specific IgE, we performed SDS-PAGE and IgE immunoblot analysis by using 3 kinds of soybean extracts and sera from 9 patients with atopic dermatitis. All patients were sensitive to soybean, which were confirmed by CAP-FEIA.
RESULTS
SDS-PAGE of non-fermented soybeans showed many bands, whereas only peptides of less than 15 kDa were found in fermented soybeans. IgE immunoblot analysis of fermented soybeans failed to detect specific IgE which were seen in non-fermented soybeans.
CONCLUSION
Fermentation could reduce the allergenicity of soybeans by efficiently degrading antigenic proteins in soybeans. However, there was no significant difference between fermentation by B. subtilis and 3-step fermentation.
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