Pediatr Allergy Respir Dis.  2001 Dec;11(4):300-309.

Analysis of Induced Sputum to Examine the Effects of Inhaled Corticosteroid on the IL-8 and Myeloperoxidase in Asthmatic Subjects

• Lee KH
• Lee JY
• Lee KM
• Lee YH
• Lee MA
• Lee SJ
• Park KS

Abstract

PURPOSE
The of this study was to describe the changes of airway inflammation in children with stable, steriod naive asthma after inhaling fluticasone by using serial induced sputum. We sought to investigate the role of neutrophils, interleukin-8(IL-8), and myeloperoxidase(MPO) in asthma. METHOD: To examine the change of neutrophil and their products in childhood asthma after inhaling fluticasone, we assessed the cell counts, IL-8, and MPO in the sputum at each visit. Also, the daily symptom scores, FEV1, peak expiratory flow variability, and methacholin airway responsiveness were checked and their relationships were analyzed. RESULT: There was a significant improvement in asthma severity, as measured by symptom score, and FEV1. There were significant reductions in IL-8 and MPO after 3 weeks treatment, and significant increase in the total sputum cell counts. Significant increase in total lymphocyte and alvelor macrophage cell counts with no increase in neutrophil and eosinophil counts were found. Airway responsiveness was significantely correlated with total eosinophil counts before inhaling fluticasone and it was correlated with IL-8 after the treatment.
CONCLUSION
This study highlight, the probable importance of IL-8 and myeloper-oxidase within the asthmatic airways and the beneficial modulatory effect of inhaled fluticasone treatment upon them.

Keyword

Bronchial asthma; Neutrophil; IL-8; Myeloperoxidase

MeSH Terms

Asthma
Cell Count
Child
Eosinophils
Humans
Inflammation
Inhalation
Interleukin-8*
Lymphocytes
Macrophages
Neutrophils
Peroxidase*
Sputum*
Fluticasone*
Interleukin-8
Peroxidase