Nutr Res Pract.  2014 Feb;8(1):59-65.

Examination of lysine requirement of healthy young male adults on a Chinese habitual diet by the modified indicator amino acid oxidation method

• Tian Y
• Peng J
• Chen Y
• Gong J
• Xu H

Abstract

There is currently no reference for intake of lysine for Chinese people; therefore, the present study was conducted to determine the lysine requirement of Chinese young male adults on a habitual Chinese mixed diet based on the modified indicator amino acid oxidation method. Seven young men with a mean age of 23.7 +/- 2.2 years that were healthy based on questionnaire, physical examinations and screening tests were evaluated. Subjects were evaluated over five consecutive 7 day periods, during which time they were administered decreasing amounts of lysine via the diet (65, 55, 45, 35, 25 mg.kg-1.d-1). Subjects were allowed to adapt from day 1 to 6 and the isotopes were measured on day 7 in each period. The subjects' body weights, body compositions and plasma proteins were also examined during the study. Amino acid kinetics were measured based on the indicator amino acid oxidation technique using the 13CO2 release rate and phenylalanine oxidation rate to estimate lysine requirements. Body weights, body compositions, and plasma proteins of subjects did not change significantly relative to those at baseline. The mean and the upper 95% CI of lysine requirements of Chinese habitual diets were determined to be 58.41 and 70.09 mg.kg-1.d-1, respectively, based on the 13CO2 release rate and 54.28 and 65.14 mg.kg-1.d-1, respectively, based on the phenylalanine oxidation rate.

Keyword

Lysine; dietary requirement; men; stable isotope

MeSH Terms

Adult*
Asian Continental Ancestry Group*
Blood Proteins
Body Composition
Body Weight
Diet*
Humans
Isotopes
Kinetics
Lysine*
Male*
Mass Screening
Nutritional Requirements
Phenylalanine
Physical Examination
Surveys and Questionnaires
Blood Proteins
Isotopes
Lysine
Phenylalanine

Figure

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  Fig. 1 δ of 13CO2 in expired air

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  Fig. 2 Effect of lysine intake on oxidation of L-[1-13C]-Phenylalanine determined from the rate of release of 13CO2 (F13CO2). The breakpoint estimates the lysine requirement. The breakpoint was determined by using 2-phase linear regression crossover analysis to minimize the total sum of squares in error for the combine line.

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  Fig. 3 Effect of lysine intake on oxidation of L-[1-13C]-Phenylalanine determined from the rate of L-[1-13C]-Phenylalanine oxidation (O). The breakpoint estimates the lysine requirement. The breakpoint was determined by using 2-phase linear regression crossover analysis to minimize the total sum of squares in error for the combine line.

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