Nutr Res Pract.  2007 Dec;1(4):273-278.

The inhibitory effect of natural bioactives on the growth of pathogenic bacteria

• Kim JS
• Kim Y

Abstract

The objective of this study was to evaluate the inhibitory activity of natural products, against growth of Escherichia coli (ATCC 25922) and Salmonella typhimurium (KCCM 11862). Chitosan, epigallocatechin gallate (EGCG), and garlic were used as natural bioactives for antibacterial activity. The testing method was carried out according to the disk diffusion method. All of chitosan, EGCG, and garlic showed inhibitory effect against the growth of E. coli and Salmonella typhi. To evaluate the antibacterial activity of natural products during storage, chicken skins were inoculated with 106 of E. coli or Salmonella typhi. The inoculated chicken skins, treated with 0.5, 1, or 2% natural bioactives, were stored during 8 day at 4degrees C. The numbers of microorganisms were measured at 8 day. Both chitosan and EGCG showed significant decrease in the number of E. coli and Salmonella typhi in dose dependent manner (P < 0.05). These results suggest that natural bioactives such as chitosan, EGCG may be possible to be used as antimicrobial agents for the improvement of food safety.

Keyword

Natural bioactives; antimicrobial activity; E. coli; Salmonella typhi

MeSH Terms

Anti-Infective Agents
Bacteria*
Biological Products
Chickens
Chitosan
Diffusion
Escherichia coli
Food Safety
Garlic
Salmonella typhi
Salmonella typhimurium
Skin
Anti-Infective Agents
Biological Products
Chitosan

Figure

• 

  Fig. 1 Effects of natural bioactives on the growth of E. coli Antimicrobial activity of various bioactives was carried out according to disk diffusion method by measuring the inhibitory zone size. a; 0%, b; 0.5%, c; 1.0%, d; 2.0% of selected bioactive compound solution

• 

  Fig. 2 Effects of natural bioactives on the growth of Salmonella typhi Antimicrobial activity of various bioactives was carried out according to disk diffusion method by measuring the inhibitory zone size. a; 0%, b; 0.5%, c; 1.0%, d; 2.0% of selected bioactive compound solution

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  Fig. 3 Inhibitory effect of chitosan and EGCG on the growth of E. coli on the chicken skin during storage at 4℃ The chicken skin surfaces (20cm2/piece) were inoculated with 106 CFU/ml of Salmonella typhi. The natural bioactives used were 0.5, 1 or 2% water soluble chitosan (A) and EGCG (B). The chicken skins treated with chitosan or EGCG were stored at 4℃ and the numbers of Salmonella typhi were counted at 8 days. The number of Salmonella typhi was expressed as mean Log10CFU/ml for the triple treatments.

• 

  Fig. 4 Inhibitory effect of chitosan and EGCG on the growth of Salmonella typhi on chicken skin during storage at 4℃ The chicken skin surfaces (20cm2/piece) were inoculated with 106 CFU/ml of Salmonella typhi. The natural bioactives used were 0.5, 1 or 2% water soluble chitosan (A) and EGCG (B). The chicken skins treated with chitosan or EGCG were stored at 4℃ and the numbers of Salmonella typhi were counted at 8 days. The number of Salmonella typhi was expressed as mean Log10CFU/ml for the triple treatments.

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