Nat Prod Sci.  2016 Mar;22(1):25-29. 10.20307/nps.2016.22.1.25.

Yield Analysis of Flavonoids in Acanthopanax divaricatus and A. koreanum Grown using Different Cultivation Methods

• Lee JM
• Lee J
• Lee JJ
• Lee SC
• Lee S

• KMID: 2312917
• DOI: http://doi.org/10.20307/nps.2016.22.1.25

Abstract

High-performance liquid chromatography was performed in order to analyze the changes in the flavonoid content (rutin, hyperin, afzelin, quercetin, and kaempferol) of Acanthopanax divaricatus and A. koreanum, in response to different cultivation methods (pinching height, planting time, and top dressing). The total flavonoid content of A. divaricatus and A. koreanum ranged from 0.201 to 0.690 mg/g with different pinching heights, 0.143 to 1.001 mg/g for different planting times, and 0.156 to 1.074 mg/g depending on the rate of fertilizer application. In both A. divaricatus and A. koreanum, the total flavonoid content in the upper section of the plant was greater than that in the lower section. These results demonstrate which cultivation methods maximize the flavonoid content of A. divaricatus and A. koreanum, and thus help to optimize flavonoid yields to improve production for nutraceutical, pharmaceutical, and cosmeceutical applications.

Keyword

Acanthopanax spp.; Culture; Flavonoid; Harvesting; HPLC

MeSH Terms

Eleutherococcus*
Chromatography, High Pressure Liquid
Chromatography, Liquid
Dietary Supplements
Flavonoids*
Methods*
Plants
Quercetin
Flavonoids
Quercetin

Figure

• 

  Fig. 1. Chemical structures of compounds 1–5.

• 

  Fig. 2. HPLC chromatograms of compounds 1–5 (A), the 50% MeOH extracts of the upper part of A. divaricatus cultivated with planting time at April 15 (B), and A. koreanum cultivated with top dressing at N-P-K (C).

Reference

(1). Boon H., Smith M.The botanical pharmacy. Quarry Press;Kingston: 1999. p. 194.

(2). Lee T. B.Coloured flora of Korea. Hyang Mun Sa;Seoul: 2003. p. 811–814.

(3). Hong C. E., Cho M. C., Jang H. A., Lyu S. Y. J.Toxicol. Sci. 2011; 36:661–668.

(4). Lyu S. Y., Park W. B.Food Sci. Biotechnol. 2008; 17:631–636.

(5). Cai X. F., Lee I. S., Dat N. T., Shen G., Kang J. S., Kim D. H., Kim Y. H.Arch. Pharm. Res. 2004; 27:738–741.

(6). Han S. B., Park S. K., Ahn H. J., Yoon Y. D., Kim Y. H., Lee J. J., Lee K. H., Moon J. S., Kim H. C., Kim H. M.Int. Immunopharmacol. 2003; 3:683–691.

(7). Perry L. M., Metzger J.Medicinal Plants of East and Southeast Asia. The MIT Press;Cambridge: 1980. p. 41.

(8). Chang S. Y., Yook C. S., Nohara T.Chem. Pharm. Bull. 1998; 46:163–165.

(9). Chang S.Y., Yook C. S., Nohara T.Phytochemistry. 1999; 50:1369–1374.

(10). Ishikura N.Phytochemistry. 1975; 14:1439.

(11). Miyakoshi M., Shirasuna K., Sawada H., Isoda S., Ida Y., Shoji J.Nat. Med. 1995; 49:218.

(12). Shirasuna K., Miyakoshi M., Mimoto S., Isoda S., Satoh Y., Hirai Y., Ida Y., Shoji J.Phytochemistry. 1997; 45:579–584.

(13). Lee S., Jung S. H., Lee Y. S., Shin K. H.Nat. Prod. Sci. 2003; 9:4–6.

(14). Kim H. Y., Lee J. M., Yokozawa T., Sakata K., Lee S.Food Chem. 2011; 126:892–895.

(15). Rho H. S., Ahn S. M., Lee B. C., Kim M. K., Ghimeray A. K., Jin C. W., Cho D. H.Bioorg. Med. Chem. Lett. 2010; 20:7534–7536.

(16). Park H. K., Park M. S., Kim T. S., Kim S., Choi K. G., Park K. H., Korean J.Crop Sci. 1997; 42:673–677.

(17). Lee J. J., Lee S. H., Park J. S., Ahn Y. S., Lee S. C., Korean J.Medicinal Crop Sci. 2013; 21:39–44.

(18). Mok S. Y., Lee J. M., Kim H. M., Lee D. G., Yoon Y. H., Cho E. J., Lee S.Nat. Prod. Sci. 2011; 17:230–233.

(19). Lee S., Park H. S., Notsu Y., Ban H. S., Kim Y. P., Ishihara K., Hirasawa N., Jung S. H., Lee Y. S., Lim S. S., Park E. H., Shin K. H., Seyama T., Hong J., Ohuchi K.Phytother. Res. 2008; 22:1552–1556.

(20). Mok S. Y., Lee S.Food Chem. 2013; 136:969–974.

(21). Kitajima J., Takamori Y., Tanaka Y.Yakugaku Zasshi. 1989; 109:188–191.

(22). Lee J. M., Lee D. G., Lee K. H., Cho S. H., Park C. G., Lee S.Nat. Prod. Sci. 2013; 19:15–19.

(23). Kim M. S., Kim S. Y., Lee D. S., Kor J.Intl. Agric. 2000; 12:298–302.

(24). Han J. S., Kim S. K., Kim S. W., Kim Y. J.Korean, J. Medicinal Crop Sci. 2001; 9:1–7.

(25). Kim P. G., Lee K. Y., Hur S. D., Kim S. H., Lee E. J.Korean J. Ecol. 2003; 26:321–326.