Nat Prod Sci.  2016 Mar;22(1):20-24. 10.20307/nps.2016.22.1.20.

Biological Activities and Stability of a Standardized Pentacyclic Triterpene Enriched Centella asiatica Extract

• Puttarak P
• Brantner A
• Panichayupakaranant P

• KMID: 2312916
• DOI: http://doi.org/10.20307/nps.2016.22.1.20

Abstract

Pentacyclic triterpenes, mainly, asiatic acid, madecassic acid, asiaticoside, and madecassoside are the active constituents of Centella asiatica. A pentacyclic triterpene enriched C. asiatica extract (PRE) was prepared and standardized to contain a total pentacyclic triterpenes not less than 65% w/w. This work was focused on determination of antiinflammatory, antioxidant, and tyrosinase inhibitory activities of PRE and its stability. The PRE exhibited a satisfactory nitric oxide inhibitory effect, with an IC50 value of 64.6 µg/mL. In addition, the PRE inhibited tyrosinase enzyme activity with an IC50 value of 104.8 µg/mL. In contrast, the PRE possessed only weak antioxidant activity. The PRE was stable over a period of four months when stored as a dried powder but only in a well-closed container protected from light at 4 °C. An aqueous alcoholic solution of the PRE was stable at pH values of 5.8 and 7.0, but was not stable at a pH of 8.2. Preparations of the PRE in an aqueous solution should be performed in acidic or neutral conditions.

Keyword

Centella asiatica; Pentacyclic triterpenes; Antiinflammatory; Antioxidant; Tyrosinase inhibition; Stability

MeSH Terms

Alcoholics
Centella*
Humans
Hydrogen-Ion Concentration
Inhibitory Concentration 50
Monophenol Monooxygenase
Nitric Oxide
Pentacyclic Triterpenes
Monophenol Monooxygenase
Nitric Oxide
Pentacyclic Triterpenes

Figure

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  Fig. 1. HPLC chromatogram of the standardized PRE. Madecassoside (1); asiaticoside (2); madecassic acid (3); asiatic acid (4).

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  Fig. 2. Effect of light on the stability of the PRE.

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  Fig. 3. Effect of temperature on the stability of the PRE.

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  Fig. 4. Effect of accelerated conditions on the stability of PRE.

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  Fig. 5. Effect of pH on the stability of PRE.

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