Mycobiology.  2011 Sep;39(3):200-205.

Biological Characterization of Marssonina coronaria Associated with Apple Blotch Disease

Affiliations
  • 1Apple Experiment Station, National Horticultural Research Institute, Gunwi 716-812, Korea.
  • 2College of Agricultural and Life Sciences, Kyungpook National University, Daegu 702-701, Korea. heeyoung@knu.ac.kr

Abstract

Marssonina coronaria associated with apple blotch disease causes severe premature defoliation, and is widely distributed in Korea. Thirteen isolates were collected from orchards located in Gyeongbuk Province from 2005~2007. All isolates displayed over 99.6% and 99.2% sequence similarity to each other in internal transcribed spacer regions and partial sequences of 28S rDNA, respectively. The isolates were phylogenetically closely related to Chinese isolates. Selected isolates did not differ in their pathogenicity. The optimum conditions for fungal growth were 20degrees C and pH 6 on peptone potato dextrose agar (PPDA). Peptone and mannose were the best nitrogen and carbon source, respectively. Fungal growth was better on PPDA than on common potato dextrose agar. This study provides valuable information for integrated disease management program and facilitates the routine culturing of M. coronaria.

Keyword

Apple blotch; Fungal growth; Marssonina coronaria; Pathogenicity; Phylogeny

MeSH Terms

Agar
Asian Continental Ancestry Group
Carbon
Diazonium Compounds
Disease Management
DNA, Ribosomal
Glucose
Humans
Hydrogen-Ion Concentration
Korea
Mannose
Nitrogen
Peptones
Phylogeny
Pyridines
Solanum tuberosum
Agar
Carbon
DNA, Ribosomal
Diazonium Compounds
Glucose
Mannose
Nitrogen
Peptones
Pyridines
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