Mycobiology.
2002 Dec;30(4):202-207.
PCR Based Detection of Phellinus linteus using Specific Primers Generated from Universal Rice Primer (URP) Derived PCR Polymorphic Band
- Affiliations
-
- 1Graduate School of Bio & Information Technology, Hankyong National University, Ansung 456-749, Korea. kanghw2@hnu.hankyong.ac.kr
- 2National Institute of Agricultural Biotechnology, RDA, Suwon 447-707, Korea.
- 3Ja-Kwang Research Institute, Hankook SinYak Pharm. Co., LTD., Nonsan 320-854, Korea.
- 4Seolin Bioscience Institute, Seolin Bldg. 452-2, Songnae-Dong, Gangdong-Gu, Seoul 134-030, Korea.
- 5Korea National Agricultural College, RDA, Suwon 445-893, Korea.
Abstract
- This study was carried out to develop specific primers for PCR detection of Phellinus linteus. Diverse genomes of 15 Phellinus spp. including five Phellinus linteus isolates were fingerprinted by Primer Universal rice primer (URP)1F. The URP-PCR pattern differentiated P. linteus isolates from other phellinus spp. A polymorphic band (2.8 kb), which is unique for P. linteus isolates, was isolated and sequenced. Twenty four-oligonucleotide primer pairs were designed based on information of DNA sequence. The primer set (PLSPF2/PLSPR1) amplified single band (2.2 kb) of expected size with genomic DNA from seven Phellinus linteus, but not with that of other Phellinus species tested. The primers could be used identically in both DNA samples from mycelium and fruit bodies. This specific primers could offer a useful tool for detecting and identifying P. linteus rapidly.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- PCR-Based Sensitive Detection of Wood-Decaying Fungus Phellinus linteus by Specific Primer from rDNA ITS Regions
- Molecular Detection of Phellinus linteus and P. baumii by PCR Specific Primer
- Development of Species-specific Primers for Rapid Detection of Phellinus linteus and P. baumii
- Genomic Differentiation Among Oyster Mushroom Cultivars Released in Korea by URP-PCR Fingerprinting
- Phylogenetic Analysis and Rapid Detection of Genus Phellinus using the Nucleotide Sequences of 18S Ribosomal RNA
