Molecular Detection of Phellinus linteus and P. baumii by PCR Specific Primer

Nam, Byung Hyouk; Kim, Gi Young; Park, Hyung Sik; Lee, Sang Joon; Lee, Jae Dong

Abstract

Specific primer sets based on ribosomal DNA (rDNA) internal transcribed specer (ITS) sequences were designed for rapid detection of Phellinus linteus and P. baumii. Polymerase chain reaction (PCR) with these primers produced unique bands for each Phellinus species. The annealing temperature range is from 40degrees C to 55degrees C. The length of PCR products (P. linteus and P. baumii) using designed combinative primer sets of PL1F, PL2R, PB1F, PB2R, ITS5F and ITS4R, were from 520 bp to 730 bp. Fifteen strains of Phellinus species including P. linteus, P. baumii, P. weirianus, P. johnsonianus, P. rhabarberinus, P. pini, P. gilvus, P. igniarius, P. nigricans and P. laevigatus were examined in this study. Five strains, including two isolated strains of P. linteus (MPNU 7001 and MPNU 7002), and two isolated strains of P. baumii (MPNU 7004 and MPNU 7005) were shown to have about 520 bp (PL1F-PL2R), 700 bp (ITS5F-PL2R) and 600 bp (PB1F-ITS4R)-sized PCR single bands respectively. This molecular genetic technique provided a useful method for rapid detection and identification of P. linteus and P. baumii.