Lab Med Online.  2012 Oct;2(4):223-225.

Chromosomal Instability in the Peripheral Blood Lymphocytes of an Ovarian Cancer Patient Undergoing Chemotherapy

Affiliations
  • 1Department of Laboratory Medicine, Ewha Womans University School of Medicine, Seoul, Korea. JungWonH@ewha.ac.kr
  • 2Department of Obstetrics and Gynecology, Ewha Womans University School of Medicine, Seoul, Korea.

Abstract

Chemotherapy agents can induce chromosomal instability, including a variety of chromatid or chromosomal aberrations. However, only limited data is available on the effect of chemotherapy on the kinetics of chromosomal instability in peripheral blood lymphocytes. Here, we report the case of an ovarian cancer patient who showed chromosomal instability in peripheral blood lymphocytes while undergoing chemotherapy. Karyotypic analysis of peripheral blood 1 day after administration of cisplatin and etoposide showed chromosomal or chromatid aberrations, including gaps, breaks, and fragmentation. Chromosome study after completion of the first chemotherapy cycle showed normal karyotype. This finding suggests that chemotherapeutic agents can induce transient chromosomal instability in peripheral blood lymphocytes.

Keyword

Chromosome; Chemotherapy; Ovarian cancer; Peripheral blood; Instability

MeSH Terms

Chromatids
Chromosomal Instability
Chromosome Aberrations
Cisplatin
Etoposide
Humans
Karyotype
Kinetics
Lymphocytes
Ovarian Neoplasms
Cisplatin
Etoposide

Figure

  • Fig. 1 G-banded karyogram 1 day after chemotherapy (cisplatin and etoposide) showing chromosomal instability, including chromatid gap (chtg) or break (chtb), chromosomal gap (chrg) or break (chrb), and fragmentation. Chtg, region of a single chromatid with minimal misalignment of the chromatid; chtb, discontinuity of a single chromatid with clear misalignment of one of the chromatids; chrg, a region at the same locus in both chromatids of a single chromosome with minimal misalignment of the chromatids; chrb, discontinuity at the same locus in both chromatids of a single chromosome, which gives rise to an acentric fragment and an abnormal monocentric chromosome.


Reference

1. Martínez-López W, Folle GA, Cassina G, Méndez-Acuña L, Di-Tomaso MV, Obe G, et al. Distribution of breakpoints induced by etoposide and X-rays along the CHO X chromosome. Cytogenet Genome Res. 2004. 104:182–187.
Article
2. Thompson SL, Bakhoum SF, Compton DA. Mechanisms of chromosomal instability. Curr Biol. 2010. 20:R285–R295.
Article
3. Lingle WL, Lukasiewicz K, Salisbury JL. Deregulation of the centrosome cycle and the origin of chromosomal instability in cancer. Adv Exp Med Biol. 2005. 570:393–421.
Article
4. Fucic A, Jazbec A, Mijic A, Seso-Simic D, Tomek R. Cytogenetic consequences after occupational exposure to antineoplastic drugs. Mutat Res. 1998. 416:59–66.
Article
5. Resende PA, Fidalgo C, Alves PM, Tavares-Murta BM, Murta EF, Dias FL. Analysis of the cytogenetic response in peripheral blood lymphocytes from breast cancer patients following chemotherapy. Eur J Gynaecol Oncol. 2010. 31:75–79.
6. Sánchez-Suárez P, Ostrosky-Wegman P, Gallegos-Hernández F, Peñarroja-Flores R, Toledo-García J, Bravo JL, et al. DNA damage in peripheral blood lymphocytes in patients during combined chemotherapy for breast cancer. Mutat Res. 2008. 640:8–15.
Article
7. Hagmar L, Brøgger A, Hansteen IL, Heim S, Högstedt B, Knudsen L, et al. Cancer risk in humans predicted by increased levels of chromosomal aberrations in lymphocytes: Nordic study group on the health risk of chromosome damage. Cancer Res. 1994. 54:2919–2922.
8. Rossner P, Boffetta P, Ceppi M, Bonassi S, Smerhovsky Z, Landa K, et al. Chromosomal aberrations in lymphocytes of healthy subjects and risk of cancer. Environ Health Perspect. 2005. 113:517–520.
Article
9. Rosselli F, Zaccaro L, Venturi M, Rossi AM. Persistence of drug-induced chromosome aberrations in peripheral blood lymphocytes of the rat. Mutat Res. 1990. 232:107–114.
Article
10. Padjas A, Lesisz D, Lankoff A, Banasik A, Lisowska H, Bakalarz R, et al. Cytogenetic damage in lymphocytes of patients undergoing therapy for small cell lung cancer and ovarian carcinoma. Toxicol Appl Pharmacol. 2005. 209:183–191.
Article
11. Stein CK. McPherson RA, Pincus MR, editors. Applications of cytogenetics in modern pathology. Clinical diagnosis and management by laboratory methods. 2011. 22nd ed. Philadelphia: WB Saunders;1311–1312.
Article
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