Lab Anim Res.  2012 Mar;28(1):61-66. 10.5625/lar.2012.28.1.61.

Molecular identification of Mycoplasma cynos from laboratory beagle dogs with respiratory disease

• Hong S
• Kim O

• KMID: 2312094
• DOI: http://doi.org/10.5625/lar.2012.28.1.61

Abstract

In this study, we examined a colony of 20 beagle dogs in a laboratory animal facility. Mycoplasma was detected by consensus PCR assay in 1 dog with respiratory and constitutional symptoms. None of the other dogs were affected. The dog was euthanized and necropsied. In postmortem examinations, gray or plum-colored gross lesions were found on the lung, most commonly in the apical and cardiac lobes. Some lesions showed clear demarcation and consolidation. Microscopic examination showed peribronchiolar lymphoid hyperplasia and interstitial thickening, lesions pathognomonic for mycoplasma pneumonia. To identify canine Mycoplasma species, we used species-specific PCR reactions for M. arginini, M. canis, M. cynos, M. edwardii, M. felis, M. gateae, M. maculosum, M. molare, M. opalescens, M. spumans, Mycoplasma sp. HRC 689, and M. collis. As the result, we identified Mycoplasma cynos by amplification of DNA extracted from lung tissue of the laboratory beagle dog with respiratory disease.

Keyword

Mycoplasma; PCR; consensus; Mycoplasma cynos; Beagle; dog

MeSH Terms

Animals
Animals, Laboratory
Autopsy
Cats
Consensus
DNA
Dogs
Felis
Hyperplasia
Lung
Molar
Mycoplasma
Pneumonia, Mycoplasma
Polymerase Chain Reaction
DNA

Figure

• 

  Figure 1 Histopathological findings in the lung of dog No. 8. (A) Peribronchiolar lymphoid hyperplasia and interstitial thickening lesions. H&E stain, Bar=100 µm, (B) Infiltrating lymphocytes and mononuclear cells in peribronchiolar hyperplasia lesion. H&E stain, Bar=20 µm

• 

  Figure 2 Amplification of 16S rDNA gene by mycoplasma consensus PCR was confirmed on a 1.2% agarose gel electrophoresis. Lane P: positive control, N: distilled water, 1~20: Nasal swab sample of No. 1~No. 20 dog

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  Figure 3 Amplification of DNA extracted from the lung tissue of dog No. 8 with canine Mycoplasma species-specific primers was confirmed on 1.2% agarose gel electrophoresis. Lane P: positive control, N: distilled water, 1: M. arginini, 2: M. canis, 3: M. cynos, 4: M. edwardii, 5: M. felis, 6: M. gateae, 7: M. maculosum, 8: M. molare, 9: M. opalescens, 10: M. spumans, 11: Mycoplasma sp. HRC 689, 12: M. collis

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