Korean J Blood Transfus.
2013 Aug;24(2):147-154.
Human Platelet Antigen Genotyping Using a Multiplex Single-Base Primer Extension Reaction in Koreans
- Affiliations
-
- 1Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. m91w95@dreamwiz.com
- 2Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
- 3Department of Laboratory Medicine, Hallym University Dongtan Sacred Heart Hospital, Hwaseong, Korea.
Abstract
- BACKGROUND
Alloimmunization of human platelet antigens (HPA) is associated with clinically significant disease, such as platelet refractoriness, neonatal alloimmune thrombocytopenia, or posttransfusion purpura. It is determined by single nucleotide polymorphism of genes for platelet membrane glycoprotein. To date, approximately 27 HPAs have been discovered, and their frequencies differ depending on ethnicity and country.
METHODS
We conducted an investigation of prevalence of HPA in the Korean population using a multiplex single-base primer extension reaction (SNaPshot). With 84 specimens from healthy donors, HPA genotyping was performed on 11 different HPAs, including HPA-1, -2, -3, -4, -5, -6, -7, -8, -9, -13, and -15.
RESULTS
A total of 90 blood samples were genotyped. The genotype frequencies of HPA were as follows: HPA-1a/1a: 100.0%, -2a/2a: 83.3%, -2a/2b: 14.3%, -2b/2b: 2.4%, -3a/3a: 39.3%, -3a/3b: 52.4%, -3b/3b: 8.3%, -4a/4a: 100.0%, -5a/5a: 95.2%, -5a/5b: 4.8%, -6a/6a: 94.0%, -6a/6b: 6.0%, -7a/7a: 100.0%, -8a/8a: 100.0%, -9a/9a: 97.6%, -9a/9b: 2.4%, -13a/13a: 100.0%, -15a/15a: 23.8%, -15a/15b: 51.2%, and -15b/15b: 25.0%.
CONCLUSION
The SNaPshot assay was employed for detection of SNPs in various clinically significant HPA genes. In addition to well-known frequencies of previously reported HPA-1 to -8, this study showed frequencies of HPA-9, -13, and -15 in Koreans for the first time. The SNaPshot technique might be suitable for use in actual clinical testing in patients with platelet alloimmunization.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- ABO Genotyping using a Multiplex Single-base Primer Extension Reaction
- The Genotype Frequencies of Platelet-Specific Antigens in Koreans
- CYP3A5 rs776746 Genotyping by SYBR GreenBased Real-Time Multiplex Polymerase Chain Reaction Combined with Melting Curve Analysis Using Tm-Shift Method
- The Preparation of Platelet Panel using DNA genotyping
- Rapid and Simple Screening of Mitochondrial DNA in Koreans by the Analysis of Highly Variable Control Region SNPs
