Korean J Blood Transfus.  1997 May;8(1):93-102.

The Genotype Frequencies of Platelet-Specific Antigens in Koreans

• Seo DH
• Park SS
• Kim DW
• Han KS

Abstract

BACKGROUND
The platelet-specific antigens which exist specifically on platelets have their antigenic determinant on platelet membrane glycoprotein. The alloantibodies against this antigens are responsible for neonatal alloimmune thrombocytopenia, posttransfusion purpura and platelet refractoriness in multitransfused patients. Discovering of the fact that the polymorphism of platelet-specific antigens is resulted from a single base pair substitution of genomic DNA stimulated studies on genotyping of platelet-specific antigens on various populations. This study was performed to investigate the genotype frequency of platelet-specific antigens in Koreans.
METHODS
Using genomic DNA extracted from venous blood of 200 Koreans, genotype of seven platelet-specific antigen systems were determined through allele-specific polymerase chain reaction and restriction fragment length polymorphism.
RESULTS
The genotype frequencies of HPA-1 were a+b- 98.0%, a+b+ 1.5%, a-b+ 0.5%; HPA-2, a+b- 85.5%, a+b+ 13.5%, a-b+ 1.0%; HPA-3, a+b- 28.5%, a+b+ 54.0%, a-b+ 17.5%; HPA-4, a+b- 98.0%, a+b+ 2.0%, a-b+ 0.0%; HPA-5, a+b- 95.5%, a+b+ 4.5%; HPA-6W, a+b- 96.0%, a+b+ 4.0%; HPA-7W were a+b- 100.0%, a+b+ 0.0%.
CONCLUSION
The gene frequency of HPA-1b in Koreans was lower than that of Caucasian. As a whole, the genotype frequencies of platelet-specific antigens in Koreans were similar to those of the Japanese. However, we found one HPA-1(a-b+) among 200 Koreans, which is very rare in Japanese. This study will serve as a basic data for the study and management of the patients with diseases associated with platelet-specific antigens and antibody reactions in Koreans.

Keyword

Platelet-specific antigen; Genotype; Allele-specific polymerase chain reaction; RFLP; Gene frequency

MeSH Terms

Antigens, Human Platelet*
Asian Continental Ancestry Group
Base Pairing
Blood Platelets
DNA
Gene Frequency
Genotype*
Humans
Isoantibodies
Membrane Glycoproteins
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Purpura
Thrombocytopenia, Neonatal Alloimmune
Antigens, Human Platelet
DNA
Isoantibodies
Membrane Glycoproteins