Korean J Blood Transfus.
2013 Aug;24(2):128-139.
Influence of Pathogen Inactivation on the Quality of Platelet Rich Plasma Derived Platelets
- Affiliations
-
- 1Blood Transfusion Research Institute, Korean Red Cross, Seoul, Korea. sykwon@redcross.or.kr
- 2Blood Service Headquarters, Korean Red Cross, Seoul, Korea.
- 3Seoul Nambu Blood Center, Korean Red Cross, Seoul, Korea.
- 4Department of Laboratory Medicine, Korea Cancer Center Hospital, Korea Institute of Radiological & Medical Sciences, Seoul, Korea.
- 5Department of Laboratory Medicine, Sanggyepaik Hospital, College of Medicine, Inje University, Seoul, Korea.
- 6Division of Vaccine Research, Center for Infectious Disease, Korea National Institute of Health, Korea Center for Disease Control & Prevention, Osong, Korea.
Abstract
- BACKGROUND
Pathogen inactivation (PI) is a proactive approach to overcome the limitations of the current testing system and donor questionnaires. Effect of PI on non-leukoreduced platelet rich plasma derived platelets (PRP-PLTs) suspended in plasma has not yet been evaluated. This study was conducted in order to evaluate the effect of PI on the quality of non-leukoreduced PRP-PLTs suspended in plasma.
METHODS
PRP-PLTs treated with the Mirasol PRT System and the Intercept Blood System were tested for PLT count, blood gas, PLT activation, and apoptosis on days 3, 5, and 7 of storage.
RESULTS
PLT number showed a decrease after PI. No difference in pH was observed until day 5. At day 7, PLTs treated with Mirasol had a lower pH value (6.5), however, it satisfied the quality control criteria. PLTs treated with Mirasol had a lower pO2 compared to pre-inactivation PLTs. pO2 during storage differed significantly between the two PI groups. pCO2 showed a decrease after inactivation and both groups showed a significant difference, compared with the control. PLTs treated with Mirasol had increased P-selectin expression after inactivation; however, difference of P-selectin during storage was not significant compared to the control. P-selectin of PLTs treated with Intercept was significantly different compared to those treated with Mirasol and control. Annexin V showed an increase after inactivation in Mirasol treated PLTs and difference during storage was significant compared to control and Intercept.
CONCLUSION
As both PI systems showed satisfactory pH values, the criteria showing a high correlation with in vivo PLT viability and generally applied to monitor quality of PLTs, quality of PRP-PLTs after PI appears not to be negatively affected.
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