Korean J Biol Psychiatry.  2005 Nov;12(2):136-142.

Effects of Venlafaxine and Dexamethasone Treatment on HSP70 Expression in Rat C6 Glioma Cells

• Yu JH
• Lee JS
• Yang BH
• Choi MR
• Chai YG
• Kim SH
• Roh SW
• Oh DY
• Choi IG

Abstract

OBJECT: The intracellular action of the antidepressant, venlafaxine, was studied in C6-gliomas using heat shock protein 70(HSP70) immunocytochemistry and HSP70 Western blots because HSP70 is associated with stress and depression.
METHODS
To examine how the glucocorticoid affects the expression of HSP70 in nerve cells, the rat C6 glioma cell was treated with dexamethasone for 6 hours. In addition, venlafaxine was administered to the experimental groups of C6 glioma cells for 1, 6, 24, and 72 hours each, after which the expression of HSP70 was investigated. Finally, venlafaxine and dexamethasone were simultaneously administered to the experimental groups for 1, 6, 24, and 72 hours, followed by an investigation of the expression of HSP70.
RESULTS
The short term(1 hour) venlafaxine treatment significantly increased the level of HSP70 expression. The short term treatment of venlafaxine with dexamethasone also increased the level of HSP70 expression but this reduction was not statistically significant. The long term(72 hours) venlafaxine with dexamethasone treatment significantly reduced the level of HSP70 expression. The long term treatment of venlafaxine also reduced the level of HSP70 expression but this reduction was not statistically significant. Dexamethasone(10uM, 6hours) did not affect the level of HSP70 expression compared with controls.
CONCLUSION
Venlafaxine increases the expression of HSP70 at short term treatment, but prolonged treatment with dexamethasone suppresses the expression of HSP70.

Keyword

Venlafaxine; Dexamethasone; Heat shock protein 70(HSP70)

MeSH Terms

Animals
Blotting, Western
Depression
Dexamethasone*
Glioma*
Heat-Shock Proteins
Immunohistochemistry
Neurons
Rats*
Venlafaxine Hydrochloride
Dexamethasone
Heat-Shock Proteins