Korean J Asthma Allergy Clin Immunol.  2007 Mar;27(1):26-32.

Comparative Evaluation of GENEDIA-ELISA Assay and MAST-Immunoblot (RIDASCREEN) Assay for Detecting Allergen-specific IgE against 16 Common Inhalant Allergens in Korea

Abstract

BACKGROUND AND OBJECTIVE
A novel multiple antigen simultaneous test using ELISA assay (GENEDIA-ELISA) has been developed. It includes two spotted spider mite (TSM) and Japanese Hop pollen (Hop J), which are currently common allergens in Korea but were not readily available for test in the past. The aim of this study was to validate GENEDIA-ELISA assay and to compare it with MAST-Immunoblot assay which was one of the commonly used in vitro tests in Korea. METHOD: One hundred eight patients were evaluated with skin prick tests, MAST-Immunoblot and GENEDIA-ELISA assay. Using skin prick test results (A/H ratio > 1) as the gold standard, the sensitivity, specificity, positive predictive value, and negative predictive value of GENEDIA-ELISA (cut-off=class 2) and MAST-Immunoblot (cut-off=class 2) were examined. The results were analyzed using receiver operating characteristic (ROC) analysis. RESULT: The overall sensitivity of GENEDIA-ELISA was higher than that of MAST-Immunoblot (61.4% vs. 38.4%; P < 0.05) while the overall specificity of each test was similar (88.5% vs. 93.9%). There was a higher correlation between GENEDIA-ELISA and skin prick tests than that obtained between MAST-Immunoblot and skin prick tests (0.47 vs. 0.39). In ROC analysis, the overall result of GENEDIA-ELISA yielded an area under the ROC curve (AUC) of 0.75, which indicated that this assay is more accurate test than MAST-Immunoblot (AUC=0.66). The sensitivities for TSM and Hop J on GENEDIA-ELISA were 83.3% and 83.3%, respectively, and AUC were 0.73 and 0.89, respectively.
CONCLUSION
GENEDIA-ELISA assay may be a sensitive and acceptable tool of specific IgE against common inhalant allergens in Korea.

MeSH Terms

Allergens*
Area Under Curve
Asian Continental Ancestry Group
Enzyme-Linked Immunosorbent Assay
Humans
Humulus
Immunoglobulin E*
Korea*
Pollen
ROC Curve
Sensitivity and Specificity
Skin
Tetranychidae
Allergens
Immunoglobulin E