Korean Circ J.  1998 Sep;28(9):1583-1589. 10.4070/kcj.1998.28.9.1583.

Effect of Carvedilol on the Growth of Vascular Smooth Muscle Cells

• Kim IS
• Park SJ
• Lim SH
• Heo YS
• Kim SW
• Kim TH
• Kim CJ
• Ryu WS
• Ryoo UH

• KMID: 2298007
• DOI: http://doi.org/10.4070/kcj.1998.28.9.1583

Abstract

Background and objectives
Carvedilol is a cardiovascular drug, beta- and alpha1-adrenoceptor antagonist, currently approved for the treatment of hypertension, angina, congestive heart failure by FDA. Carvedilol has been shown to attenuate oxygen free radical-initiated lipid peroxidation and to inhibit neointimal formation of aorta following vascular injury by balloon angioplasty. We have investigated the effect of carvedilol on DNA synthesis of vascular smooth muscle cells (VSMC) stimulated by platelet-derived growth factor (PDGF)-BB. MATERIALS AND METHOD: Rat aortic smooth muscle cells were obtained by the combined collagenase and elastase methods. Cells between the 4th and 8th passages were used for the experiments. Incorporated radioactivity of [3H]-thymidine was measured by liquid scintillation spectrometry.
RESULTS
PDGF-BB (1 nM) increased [3H]-thymidine incorporation about 70-100% over basal value in cultured VSMC. PDGF-stimulated increase in DNA synthesis was significantly suppressed by simultaneous administration of carvedilol. In contrast, propranolol did not significantly affect 3[H]-thymidine uptake in rat aortic VSMC.
CONCLUSION
The present study demonstrate that carvedilol significantly inhibits the proliferation of vascular smooth muscle cell in our condition. These results indicate that carvedilol may be effective in the treatment of cardiovascular diseases principally associated with abnormal vascular smooth muscle growth.

Keyword

Carvedilol; Vascular smooth muscle cell; Cell proliferation

MeSH Terms

Angioplasty, Balloon
Animals
Aorta
Cardiovascular Diseases
Cell Proliferation
Collagenases
DNA
Heart Failure
Hypertension
Lipid Peroxidation
Muscle, Smooth, Vascular*
Myocytes, Smooth Muscle
Oxygen
Pancreatic Elastase
Platelet-Derived Growth Factor
Propranolol
Radioactivity
Rats
Spectrum Analysis
Vascular System Injuries
Collagenases
DNA
Oxygen
Pancreatic Elastase
Platelet-Derived Growth Factor
Propranolol