Korean J Urol.  2005 May;46(5):518-525.

HIF-1alpha, iNOS and VEGF Expression of Contralateral Kidney during Acute Stage in Complete Ureteral Obstruction of Rat Model

• Nam JK
• Lee SD
• Kim BO

Abstract

Purpose
To understand the process of hemodynamic change and compensatory hypertrophy of the contralateral kidney in an acute ureteral obstruction (UO), the inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1alpha (HIF-1alpha) expressions in the contralateral kidney were evaluated during the acute stage of unilateral UO in the rat.
Materials and Methods
Specimens (kidneys) were aseptically removed from 35 Sprague-Dawley rats (8 weeks old, weight 250-300gm) of the control (0 hours), sham (6, 12 and 24 hours) and experimental groups (UO 6, 12 and 24 hours). The iNOS, VEGF and HIF-1alpha expressions in the contralateral kidney (total renal tissue, medulla and cortex) were evaluated by immunohistochemical staining. The HIF-1alpha expression was also evaluated by a Western blot assay.
Results
Contrary to the control and sham groups, the iNOS, VEGF and HIF-1alpha expressions in the contralateral kidney from the immunohistochemical staining increased with time of obstruction, especially in the medulla. The HIF-1alpha expression in the contralateral kidney by the Western blot assay was increased in all the renal tissue, medulla and cortex compared to those in the control and sham groups.
Conclusions
In the contralateral kidneys of rats with UO increased expressions of iNOS, VEGF and HIF-1alpha may facilitate trophic changes and a compensatory increase in the blood flow.

Keyword

Ureteral obstruction; Kidney; HIF-1alpha; Inducible nitric oxide synthase; VEGF

MeSH Terms

Animals
Blotting, Western
Hemodynamics
Hypertrophy
Kidney*
Models, Animal*
Nitric Oxide Synthase Type II
Rats*
Rats, Sprague-Dawley
Ureter*
Ureteral Obstruction*
Vascular Endothelial Growth Factor A*
Nitric Oxide Synthase Type II
Vascular Endothelial Growth Factor A