Korean J Urol.  1998 Nov;39(11):1051-1060.

Establishment and Characterization of New Human Renal Oncocytoma Cell Line(CURO)

• Moon SY
• Moon WC

Abstract

PURPOSE: Renal oncocytoma has been a focus of interest in urologic oncology. The biologic and molecular characteristics of this disease remains ill defined due to paucity of ideal in vitro model. In this present study a new cell line of human renal oncocytoma, CURO, has been established and characterized. MATERIALS AND METHOD: The CURO cells were cultured from tissues obtained from radical nephrectomy specimen of incidentally found renal oncocytoma. The cellular and molecular biological characleristics of CURO cells were analyzed.
RESULTS
CURO cells grew in monolayer with a rapid doubling time of 20 hours. The cells showed abundant mitochondria and well developed microvilli, and expressed cytokeratin, epithelial membrane antigen, and lectins of distal renal tubular and collecting duct origin. The cells showed aneuploidy with high proportion of cells in G2+M phase(27%) on flow cytometric analysis. Karyotyping study revealed clonal heterogeneity: Majority showed normal 46XX, whereas, 12% of cells showed deletion or translocation of chromosome 19, but none of the cells showed abnormality of 3p. The cells neither showed mutation of p53 gene and nor expressed two major angiogenic factors of renal cell carcinoma: vascular endothelial growth factor and basic fibroblast growth factor. The CURO cells didn't show tumorigenecity in athymic nude mouse on either subcutaneous or subrenal capsular implantation.
CONCLUSIONS
The results of this study suggest that CURO may be a valuable model to study renal oncocytoma. Renal oncocytoma may be a benign tumor of distal renal tubular or collecting duct origin, but it may contain clone with high proliferative activity. Change of chromosome 19 may be a marker of development or proliferation of renal oncocytoma.

Keyword

Renal oncocytoma; Cell line; Establishment; Characterization

MeSH Terms

Adenoma, Oxyphilic*
Aneuploidy
Angiogenesis Inducing Agents
Animals
Carcinoma, Renal Cell
Cell Line
Chromosomes, Human, Pair 19
Clone Cells
Fibroblast Growth Factor 2
Genes, p53
Humans*
Karyotyping
Keratins
Lectins
Mice
Mice, Nude
Microvilli
Mitochondria
Mucin-1
Nephrectomy
Population Characteristics
Vascular Endothelial Growth Factor A
Angiogenesis Inducing Agents
Fibroblast Growth Factor 2
Keratins
Lectins
Mucin-1
Vascular Endothelial Growth Factor A