Effect of Hyperthermia on Growth of Renal Cell Carcinoma Cell Lines in vitro

Lee, Jeong Heang; Lee, Eun Sik; Park, Won Hee

Abstract

PURPOSE: Recently, the cellular biology of response to hyperthermia was understood and localizing hyperthermic equipments were developed. And the results of hyperthermic treatment were reported in several malignant tumors, but the effect of hyperthermia on advanced renal cell carcinoma(RCC) is poorly understood. We investigated the effect of hyperthermia on growth and DNA synthesis of RCC cell line in vitro.
MATERIALS AND METHODS
Human renal cell carcinoma cell line, Caki-1, was used. Hyperthermia(41.5 degrees C and 43 degrees C) was applied with various duration(1, 2, and 4 hours). The cell growth was measured by MTT colorimetric assay. The 24-hour DNA synthesis was studied with 3H-thymidine uptake test. The effect of hyperthermia on cell-cycle was analysed by flowcytometry.
RESULTS
In 2-hour treatment with 41.5 degrees C or 43 degrees C of hyperthermia, the cell growth was inhibited up to 25% and in 4-hour treatment up to 50% The DNA synthesis was inhibited as similar pattern of cell growth. In flowcytometry, the decrease of S-phase fraction in hyperthermia treated cells was recognized in dose and time dependent manner.
CONCLUSIONS
These results suggest that the localizing hyperthermia could be applied In advanced renal cell carcinoma patients for cytoreductive purpose because there are yet no definitive treatment modalities.