Flow cytometric DNA ploidy and proliferating cell nuclear antigen in prostatic adenocarcinoma

Seong, In Gi; Yoon, Jong Byung

Abstract

Clinical significance of flow cytometric DNA ploidy and proliferating cell nuclear anrigen (PCNA) was evaluated in terms of clinical stage. histological grade and tumor markers. using the materials obtained from paraffin embedded blocks of 47 patients with prostatic adenocarcinoma. The incidence of DNA aneuploidy in total population was 51.1 %. Although no significant correlation between histological grade or clinical stage and DNA ploidy pattern was demonstrated, the frequency or aneuploidy was shown to increase as the poorer the histological grade and the higher the clinical stage. All patients in aneuploidy group and 66.7% of the patients in diploidy group had PSA levels of more than 4ng/ml, and 57.1% of those in aneuploidy group and 50% of those in diploidy group had PAP levels of more than 3.2ng/mI. Overall, the difference in survival curves for diploidy and aneuploidy group was not significant. But. in patients of stage D with intermediate histological grade, the survival difference between diploid and aneuploidy tumors was obvious. The PCNA related proliferating index was significantly increased with the progression of the clinical stage. And the proliferating index was inversely related to the degree of glandular differentiation. but without statistical significance. Although proliferating index of prostatic adenocarcinoma didn`t have any significant correlation with the survival, the statistically significant difference was shown in survival between PCNA score+/-group and PCNA score + to +++ group.