Korean J Pediatr.  2013 Aug;56(8):332-337. 10.3345/kjp.2013.56.8.332.

Early-onset sepsis in a neonatal intensive care unit in Beni Suef, Egypt: bacterial isolates and antibiotic resistance pattern

• Fahmey SS

• KMID: 2284805
• DOI: http://doi.org/10.3345/kjp.2013.56.8.332

Abstract

PURPOSE
To identify the frequency of bacterial isolates in early-onset neonatal sepsis (EONS) and their antimicrobial resistance pattern.
METHODS
A retrospective study of EONS was conducted at the Beni Suef University Hospital from September 2008 to September 2012. A case of EONS was defined as an infant who had clinical signs of infection or who was born to a mother with risk factors for infection, and in whom blood culture obtained within 72 hours of life grew a bacterial pathogen.
RESULTS
Of 673 neonates screened, there were 138 positive blood cultures (20.5%) (confirmed EONS). Of the recovered isolates, 86.2% were gram-negative pathogens. Klebsiella pneumoniae (42.8%), Enterobacter cloacae (22.5%), and Escherichia coli (13.8%) were the commonest isolated organisms. The most common gram-positive microorganism was Staphylococcus aureus accounting for only 12 isolates (8.7%). All Klebsiella isolates and 93% of Enterobacter isolates were resistant to ampicillin. Gram-negative pathogens had the maximum overall sensitivity to imipenem, cefepime, and ciprofloxacin; whereas, gram-positive isolates were most susceptible to vancomycin, imipenem, and piperacillin.
CONCLUSION
K. pneumoniae was the predominant causative bacteria of EONS followed by E. cloacae and E. coli. There was a high resistance to ampicillin. Imipenem had the maximum overall activity against the causative bacteria. Continuous surveillance is needed to monitor the changing epidemiology of pathogens and antibiotic sensitivity.

Keyword

Drug Resistance; Newborn; Sepsis

MeSH Terms

Accounting
Ampicillin
Bacteria
Cephalosporins
Cloaca
Drug Resistance
Drug Resistance, Microbial
Enterobacter
Enterobacter cloacae
Escherichia coli
Humans
Imipenem
Infant
Infant, Newborn
Intensive Care, Neonatal
Klebsiella
Klebsiella pneumoniae
Mothers
Organothiophosphorus Compounds
Pneumonia
Retrospective Studies
Risk Factors
Sepsis
Staphylococcus aureus
Vancomycin
Ampicillin
Cephalosporins
Imipenem
Organothiophosphorus Compounds
Vancomycin