Korean J Prev Med.  2002 Nov;35(4):269-274.

Effect of Glutathione on Lead Induced Modulation of NO Synthesis in RAW 264.7 Cell

• Shin SR
• Oh GJ
• Kwon KS
• Yoon WH

Abstract

OBJECTIVES
To evaluate the effect of glutathione(GSH) on lead induced modulation of nitric oxide(NO) synthesis, and to examine how lead modulates NO production in macrophages.
METHODS
This study was observed in a culture of RAW 264.7 cells, which originated from a tumor in a Balb/c mouse that was induced by the Abelson murine leukemia virus. The compounds investigated were lead chloride, N-acetyl-cystein(NAC), and Buthionine Sulfoximine(BSO). RESUJLTS: ATP synthesis in RAW 264.7 cells was unchanged by each lead concentration exposure in a dose dependent manner. The NO synthesis was decreased when exposed to lead(PbCl2) concentration 0.5 micro M. The presence of 300 micro M NAC, used as a pretreatment in the culture medium, caused the recovery of the lead induced decrease in NO synthesis, but in the presence of 300 micro M BSO as a pretreatment, there was no recoverey. Pretreatment with NAC and BSO had no affect on ATP synthesis at any of the lead concentrations used.
CONCLUSIONS
These results indicated that GSH has a protective effect toward lead toxicity, and suggested that the inhibition of NO production in macrophage due to lead toxicity may be related to cofactors of iNOS (inducible nitric oxide synthase)

Keyword

Glutathione; Lead; Buthionine sulfoximine; N-acetylcysteine; Nitric oxide

MeSH Terms

Abelson murine leukemia virus
Acetylcysteine
Adenosine Triphosphate
Animals
Buthionine Sulfoximine
Glutathione*
Macrophages
Mice
Nitric Oxide
Acetylcysteine
Adenosine Triphosphate
Buthionine Sulfoximine
Glutathione
Nitric Oxide