Korean J Otolaryngol-Head Neck Surg.  2004 Jun;47(6):507-514.

Effect of Inhibitor of TNF-alpha and Oxatomide on Experimental Immune Mediated Otitis Media with Effusion

• Auo HJ
• Park YS
• Jeon EJ
• Yeo SW
• Chang KH
• Lee SK

Abstract

BACKGROUND AND OBJECTIVES
Local immune response, one of the pathogenesis of middle ear effusion (MEE) is associated with the development and persistence of effusion in the middle ear cavity and inflammatory mediators play a major role in the production of MEE. The purpose of this study was to determine the effects of TNF-alpha antagonist and oxatomide on the outcome of immune mediated otitis media with effusion (OME) in rats. MATERIALS AND METHOD: Otitis media was induced by injecting KLH (Keyhole lympet hemocyanin) transtympanically 7 days after systemic immunization. Phosphate-buffered saline solution was used as control. Other groups were pretreated with TNF-alpha antagonist (soluble TNF receptor type I, sTNF RI) or oxatomide respectively before transtympanic injection of KLH. Seventy-two hours after the transtympanic injection, temporal bones in each group were examined histopathologically and vascular permeability of the middle ear mucosa was measured by the Evans blue vital dye technique. RESULTS: In the KLH, sTNF RI and oxatomide groups, MEE was developed in 83%, 0%, 66% of the ears, respectively. The oxatomide group and sTNF RI group showed significant decrease in inflammation, mucosal thickening and vascular permeability as compared with KLH group and those parameters of sTNF RI group showed lower values than those of oxatomide group. CONCLUSION: Transtympanic administration of sTNF RI and oxatomide appears to suppress the development of immune mediated OME. In terms of inhibiting MEE, sTNF RI was more effective than oxatomide. This study suggests that TNF-alpha antagonist and oxatomide may have a adjunctive role in the treatment and prevention of otitis media with effusion.

Keyword

Otitis media with effusion; Tumor necrosis factor receptor; Hemocyanin; Leukotriene antagonists

MeSH Terms

Animals
Capillary Permeability
Ear
Ear, Middle
Evans Blue
Hemocyanin
Immunization
Inflammation
Leukotriene Antagonists
Mucous Membrane
Otitis Media with Effusion*
Otitis Media*
Otitis*
Rats
Receptors, Tumor Necrosis Factor
Sodium Chloride
Temporal Bone
Tumor Necrosis Factor-alpha*
Evans Blue
Hemocyanin
Leukotriene Antagonists
Receptors, Tumor Necrosis Factor
Sodium Chloride
Tumor Necrosis Factor-alpha