Korean J Pathol.  2005 Apr;39(2):81-90.

Effect of Antenatal Dexamethasone Treatment on Neuronal Morphogenesis

• Yoon SJ
• Koo H
• Kim CI

Abstract

BACKGROUND: Glucocorticoids (GCs) are essential for normal development and the maturation of the central nervous system. The aim of this study was to determine the effects of antenatal dexamethasone (DEXA) treatment on neuronal morphogenesis and on the glial cell line-derived neurotrophic factor (GDNF) protein expression in neonatal rat.
METHODS
Pregnant Sprague-Dawley rats were injected with saline (the control), or 0.2 mg/kg/day DEXA or 0.8 mg/kg/day DEXA at 17th, 18th and 19th day of gestation. The newborn rat brains were examined at postnatal days 1 (n=75) and 10 (n=78).
RESULTS
The DEXA-treated groups showed distorted architectures of neurons in the cerebral cortex, hippocampus and cerebellar cortex at postnatal days 1 and 10 with an increased number of proliferating cell nuclear antigen (PCNA)-positive cells. The cerebellar cortex in the DEXA-treated groups showed delayed development with more PCNA-positive cells in the internal granular cell layer. The Purkinje cells showed a markedly decreased number and the decreased length of the dendritic processes. The GDNF positive reaction was decreased in the DEXA-treated groups in a dose-dependent manner.
CONCLUSIONS
The developmental changes and neuronal degeneration at postnatal days 1 and 10 in the newborn rats that were exposed to DEXA at the late gestational age were associated with increased proliferative activity and a decreased level of GDNF protein expression.

Keyword

Dexamethasone; Brain; Proliferating cell nuclear antigen; Glial cell line-derived neurotrophic factor

MeSH Terms

Animals
Brain
Central Nervous System
Cerebellar Cortex
Cerebral Cortex
Dexamethasone*
Gestational Age
Glial Cell Line-Derived Neurotrophic Factor
Glucocorticoids
Hippocampus
Humans
Infant, Newborn
Morphogenesis*
Neurons*
Pregnancy
Proliferating Cell Nuclear Antigen
Purkinje Cells
Rats
Rats, Sprague-Dawley
Dexamethasone
Glial Cell Line-Derived Neurotrophic Factor
Glucocorticoids
Proliferating Cell Nuclear Antigen