Role of Stem Cell Factors in Granulation Recovery on Mast Cells of Rat

Kim, Woo Seok; Cho, Joong Saeng; Lee, Kun Hee; Pyo, Si Young; Cha, Chang Il

Abstract

BACKGROUND AND OBJECTIVES: Stem cell factor (SCF) is produced by several cell types including stromal cells in the bone marrow and fibroblasts in tissues. Formation of granules in developing mast cells is similar to the recovery of granules in the degranulated mast cells. SCF plays an important role in differentiation of mast cells from the progenitor cells, and may involve recovery of granules in degranulated mast cells. This study was carried out to verify the role of SCF in recovery of granules in the degranulated cells.
METHODS
Mast cells were separated from the peritoneal and pleural cavities of Wistar rats. The cells were degranulated by stimulation with compound 48/80. A group of degranulated mast cells were cultured with SCF for 40hrs. The contents of histamine in the supernatant and pellet were measured by ELISA.
RESULTS
The degranulated mast cells cultured with SCF had more histamine in both supernatant and pellet than in those cultured without any treatment nor stimulated with compound 48/80 for 40hours. In the cultured degranulated cells, the SCF treated cells showed more histamine contents in the pellet than in supernatant; however, the contents of these cells in the pellets were less than those cultured with SCF.
CONCLUSIONS
This study may verify that stem cell factor plays an important role in the granule recovery of degranulated mast cells.