Korean J Obstet Gynecol.  2008 May;51(5):510-517.

Differential gene expression of transglutaminase 2 in term pregnancy with and without labor

• Kim OK
• Kim A
• Seo MW
• Ahn HY
• Yang DE
• Lee GS
• Shin JC

Abstract

OBJECTIVE
Our purpose was to investigate transglutaminase 2 (TGM2) mRNA and protein expressions in term placentas and fetal membranes delivered with labor compared to no labor.
METHODS
Samples were obtained from five cases delivered with labor and five cases delivered without labor after 38 weeks of gestation. Each sample was collected from amnion, chorion, central and peripheral portion of the basal plate of placenta. Real time polymerase chain reaction (RT-PCR) was done to analyze mRNA expression of TGM2. Western blot was done and TGM2 protein level was detected. Mann-Whitney U test was used for statistic analysis.
RESULTS
In labor group, TGM2 mRNA expressions were decreased compared to no labor group in 3 sampling sites except chorion (0.66+/-0.10 vs 1.29+/-0.12 in peripheral placenta, 0.67+/-0.23 vs 1.02+/-0.02 in central placenta, 0.70+/-0.16 vs 1.04+/-0.05 in amnion in contrast with 1.62+/-0.64 vs 1.56+/-0.21 in chorion). TGM2 protein expressions of four differential portions were decreased in all labor groups (1.05+/-0.35 vs 1.27+/-0.19 in peripheral placenta, 0.69+/-0.84 vs 0.84+/-0.31 in central placenta, 0.33+/-0.15 vs 0.39+/-0.33 in amnion, 0.96+/-0.18 vs 1.77+/-0.61 in chorion).
CONCLUSIONS
This result suggests that TGM2 might involve in labor progress of term pregnancy.

Keyword

Transglutaminase 2; Labor; Pregnancy

MeSH Terms

Amnion
Blotting, Western
Chorion
Extraembryonic Membranes
Gene Expression
GTP-Binding Proteins
Placenta
Pregnancy
Real-Time Polymerase Chain Reaction
RNA, Messenger
Transglutaminases
GTP-Binding Proteins
RNA, Messenger
Transglutaminases