Korean J Obstet Gynecol.  2005 May;48(5):1271-1281.

Effects of Vero Cells Co-culture System on The In Vitro Development of Mouse Preimplantation Embryos in Media with Different Composition of Glucose and Pyruvate

• Kim JH
• Lee KH
• Son SK
• Song HB
• Kang KC

Abstract

OBJECTIVE
The purpose of this study was to examine the effects of vero cells co-culture system on the in vitro development of mouse preimplantation embryos in culture media with different composition of glucose and pyruvate.
METHODS
Two-cell embryos were collected from 4-5 weeks old ICR mice. Seven hundreds twenty two embryos were cultured with or without vero cells monolayer in four media with different compositions that was manufactured by two DMEM media with (DMEM-GGP) or without (DMEM-G) glucose and pyruvate. In control, DMEM-G medium which is currently using for human embryo culture in our infertility clinic was used. Group I (DMEM-G(1/4)GP) was cultured in medium which was mixed three volume of DMEM-G and one volume of DMEM-GGP, and group II (DMEM-G(1/2)GP) was cultured in medium which was mixed same volume of DMEM-G and DMEM-GGP, and group III was cultured in DMEM-GGP. All media were added to 20% hFF. Results between different groups were analyzed using a Chi-square test, and considered statistically significant when p value was less than 0.05.
RESULTS
The developmental rate into 3-cell CONCLUSION
In culture system using only each different DMEM media without vero cell, group II was more effective for the embryonic development than other groups. However, in co-culture system by each different DMEM media with vero cell, group III was more effective than other groups. These results are likely that was caused by consumption on glucose and pyruvate of vero cells in culture media. Therefore, the composition of energy sources in culture media may be very important point in co-culture system.

Keyword

Co-culture; Mouse preimplantation embryo; Vero cells; DMEM

MeSH Terms

Animals
Blastocyst*
Coculture Techniques*
Culture Media
Embryonic Development
Embryonic Structures
Female
Glucose*
Humans
Infertility
Mice*
Mice, Inbred ICR
Pregnancy
Pyruvic Acid*
Vero Cells*
Culture Media
Glucose
Pyruvic Acid