Korean J Obstet Gynecol.
2004 Dec;47(12):2424-2429.
Insulin-like growth factor binding protein-3 protease profiles in sera of patients with uterine myoma before and after hysterectomy
- Affiliations
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- 1Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
Abstract
OBJECTIVE
To investigate change in the activity of insulin-like growth factor binding protein-3 (IGFBP-3) protease and change in serum IGFBP-3 levels measurable by western ligand blot and immunoradiometric assay after hysterectomy, in patients with uterine myoma.
METHODS
IGFBP-3 was analyzed by Western ligand blot (WLB) and immunoradiometric assay in sera obtained from patients (n=14) with uterine myoma undergoing hysterectomy, and IGFBP-3 protease activity were measured by protease assay using [125I] IGFBP-3 as substrate. Patients (n=13, control patients) undergoing tubal reanastomosis were served as controls.
RESULTS
No significant differences in the preoperative and postoperative levels of serum IGFBP-3, and IGBPF-3 protease activity between patients with myoma and control patients were noted. In both group, serum IGFBP-3 level measured by WLB decreased significantly on 2nd day after operation and restored toward the preoperative level on 6th day after operation, while there was no significant change in postoperative serum IGFBP-3 levels measured by immunoradiometric assay. Serum IGFBP-3 protease activity increased significantly on 2nd day after operation and returned to the preoperative level on 6th day after operation in both group.
CONCLUSION
Circulating IGFBP-3 levels measurable by immunoradiometric assay does not change postoperatively and an increase in IGFBP-3 protease activity on 2nd day after operation result in a decrease in circulating IGFBP-3 levels measurable by WLB.
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