The effect of static magnetic fields on molecular and cellular activities

Park, Jae Gu; Hwang, Hyeon Shik

Abstract

Optimal force for orthodontic treatment is the force that produces a rapid rate of tooth movement without discomfort to the patient or ensuing tissue damage. Recently considerable interest has been generated in the application of magnets as a way to obtain an optimal force. The purpose of the present study was to investigate the effect of static magnetic fields of Sm-Co magnets on molecular and cellular activities. The distance of erythrocyte sedimentation was measured directly, and the acticities and the ayntheses of Fe2+ -related enzymes (xatalase and NO synthase) and non Fe2+ -related enzyme (lactic dehydrogenase) were assayed by the spectrophotometer. The growth and the proliferation of osteoblast-like cells MC3T3-E1 were determined by the crystal violet staining and the 3H-thymidine incoroiration. The erythrocytes were exposed to the pole face flux density of 1,400 G(gauss), and the enzymes and osteoblast-like cells MC3T3-E1 were exposed to the flux density of 7,000 G. The results obtained were as follows: 1. The distance of sedimentation of erythrocyte was not affected by the static magnetic fields. 2. The activities of catalase and lactic dehydrogenase were not affected by the static magnetic fields. 3. The intracellular syntheses of NO synthase and latic dehydrogenase were not affected by the static magnetic fields. 4. The growth and the proliferation if cultured osteoblast-like cells MC3T3-E1 were not affected by the static magnetic fields. There results suggested that the molecular and cellular activities were not significantly influenced by the static magnetic fields.