Korean J Obstet Gynecol.  2001 Feb;44(2):252-257.

Chromosomal Abnormality of Spontaneous Abortus in Relation to Transvaginal Ultrasonographic Finding

• Shin KJ
• Whang JD
• Yoo JK
• Han KS
• Oh YL
• Roh CR
• Park CS
• Bae DS
• Lee JH

Abstract

OBJECTIVE
To determine the clinical value of human papillomavirus deoxyribonucleic acid(HPV DNA) testing by polymerase chain reaction(PCR), specifically to examine whether HPV testing could identify the women with Pap smears read as mostly atypical squamous cells of undetermined significance(ASCUS) or more.
METHODS
HPV DNA testing by PCR for 3 high-risk cancer associated genotypes(HPV 16, 18, 33), repeat Pap smears and colposcopically directed punch biopsies were performed concurrently on 169 women referred for cervical cancer screening test with a previous Pap smear read as ASCUS or more.
RESULTS
HPV DNA testing positivity was significantly associated with abnormal cytology and high-grade squamous intraepithelial lesion(HSIL) and squamous cell carcinoma(SCC) in histology(P=0.034). The sensitivity, specificity, positive predictive value(PPV), negative predictive value(NPV) of Pap smear and HPV testing for identifying 38 cases of histologically confirmed HSIL and carcinoma by different triage protocols(HPV positive or HSIL or SCC) among 169 women were 65.8%(25/38), 85.5%(112/131), 56.8%(25/44) and 89.6%(112/125), respectively. Also sensitivity, specificity, PPV and NPV were varied by ages and more higher in older.
CONCLUSION
HPV DNA testing by PCR appears to offer an effective means by which women whose cervical Pap smears have been read as ASCUS or more could be triaged for colposcopically directed biopsy. The sensitivity for HSIL could be maintained in high and specificity markedly improved by HPV genotypes 16, 18, 33.

Keyword

Human papillomavirus deoxyribonucleic acid(HPV DNA); Pap smear; Atypical squamous cells of undetermined significance(ASCUS); High-grade squamous intraepithelial lesion(HSIL)

MeSH Terms

Biopsy
Chromosome Aberrations*
Female
Genotype
Human Papillomavirus DNA Tests
Humans
Mass Screening
Polymerase Chain Reaction
Sensitivity and Specificity
Triage
Uterine Cervical Neoplasms