Korean J Nutr.  2003 Dec;36(10):990-996.

Effect of Forsythia Viridissima Extracts on Antioxidative System and Lipid Peroxidation of Liver in Rats Fed High-Cholesterol Diet

• Lee JM
• Choi SW
• Cho SH
• Rhee SJ

Abstract

This study was conducted to investigate the effects of Forsythia viridissima Lindl. (FVL) on antioxidative defense system and lipid peroxidation of liver in rats fed high-cholesterol diet. Sprague-Dawley male rats weighing 100+/-10 g were randomly assigned into five experimental groups fed 0.5% cholesterol ; HC group which was not supplemented FVL extract, 0.05% methanol extract diet group (MSI group), 0.1% methanol extract diet group (MS2 group), 0.025% ethylacetate-souble fraction diet group (ES1 group) and 0.05% ethylacetate-souble fraction diet group (ES2 group). Experimental diets were fed ad libitum to the rats for 3 weeks. The hepatic xanthine oxidase (XOD) activity in the MS2 group was decreased to 20% as compared to HC group. The activities of hepatic superoxide dismutase (SOD) and catalase (CAT) were not significantly different among all the high cholesterol diet groups. The hepatic glutathione peroxidase (GSHpx) activity in MS2, ES2 groups were significantly increased as compared to HC group. The hepatic glutathione S-transferase (GST) activity in the MS2 group was increased to 20% as compared to HC group. The levels of hepatic TBARS in the MS1, MS2, ES1 and ES2 groups were reduced by 13%, 21%, 13% and 21%, respectively, as compared with HC group. The contents of lipofuscin in liver tissue was not significantly different among all the experimental groups. The results indicate that FVL extract may reduce oxidative damage by activating antioxidative defense system of liver in rats fed high-cholesterol diets.

Keyword

Forsythia viridissima Lindl; extract; high cholesterol diet; antioxidative system; lipid peroxidation

MeSH Terms

Animals
Catalase
Cholesterol
Diet*
Forsythia*
Glutathione Peroxidase
Glutathione Transferase
Humans
Lipid Peroxidation*
Lipofuscin
Liver*
Male
Methanol
Rats*
Rats, Sprague-Dawley
Superoxide Dismutase
Thiobarbituric Acid Reactive Substances
Xanthine Oxidase
Catalase
Cholesterol
Glutathione Peroxidase
Glutathione Transferase
Lipofuscin
Methanol
Superoxide Dismutase
Thiobarbituric Acid Reactive Substances
Xanthine Oxidase