Korean J Obstet Gynecol.
1999 May;42(5):981-989.
Clinical Efficacy of Assisted Hatching Using Acid Tyrode`s Solution in Human Preimplantation Embryos
Abstract
OBJECTIVE
To investigate the clinical efficacy of assisted hatching[AH] using acid Tyrode`s solution in in vitro fertilization and embryo transfer[IVF-ET] patients who had previous repeated failure cycles > or = 3[Group I ; 66 cycles in 56 patients], age of > or = 37 years[Group II ; 38 cycles in 32 patients], or basal serum FSH level > or = 15 mIU/ml[Group III ; 14 cycles in 12 patients].
MATERIALS AND METHODS
From January to December, 1997, a total of 75 IVF-ET cycles in 65 patients was enrolled in this descriptive study. In preimplantation embryos, AH was performed with the application of acid Tyrode`s solution on zona pellucida to create an opening just before ET.
RESULTS
The number of oocytes retrieved after controlled ovarian hyperstimulation[COH] was 8.5+/-5.3[mean+/-SD] in Group I, 7.9+/-6.2 in Group II, and 6.8+/-4.7 in Group III. The number of embryos transferred after AH was 4.6+/-2.0, 3.9+/-1.9, and 3.6+/-1.9, respectively, and the mean cumulative embryo score[CES] was 88.7+/-49.2, 80.3+/-45.0, and 78.9+/-59.6, respectively. There were no significant differences in the numbers of oocytes retrieved and embryos transferred, and CES among three groups. The clinical pregnancy rates per cycle and per patient were not different significantly among three groups : 13.6%[9/66] and 16.1%[9/56] in Group I, 13.2% [5/38] and 15.6%[5/32] in Group II, and 14.3%[2/14] and 16.7%[2/12] in Group III.
CONCLUSION
AH of human preimplantation embryos using acid Tyrode`s solution might be promising in the improvement of pregnancy rates for IVF-ET patients, especially with the previous history of repeated failure, old age, or high basal serum FSH level. Further prospective and randomized study is necessary to determine if the micromanipulation procedure of AH would be beneficial to IVF-ET patients.
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