Clin Exp Reprod Med.  2012 Sep;39(3):118-124. 10.5653/cerm.2012.39.3.118.

Outcomes of preimplantation genetic diagnosis using either zona drilling with acidified Tyrode's solution or partial zona dissection

• Kim HJ
• Kim CH
• Lee SM
• Choe SA
• Lee JY
• Jee BC
• Hwang D
• Kim KC

• KMID: 2167510
• DOI: http://doi.org/10.5653/cerm.2012.39.3.118

Abstract

OBJECTIVE
To review the outcomes of preimplantation genetic diagnosis (PGD) using zona drilling with acid Tyrode's solution (chemical zona pellucida drilling, chemical ZD) and those of partial zona dissection (PZD).
METHODS
Clinical outcomes of seventy-one couples undergoing 85 PGD cycles from January 2005 to December 2010 were included. Blastocyst formation and the hatching rate, clinical pregnancy rate, ongoing pregnancy rate, implantation rate, and fetal gender ratio of the PZD and chemical ZD groups were compared.
RESULTS
Application of PZD resulted in a significantly higher rate of clinical pregnancy (40.7% vs. 15.4%, p=0.022), ongoing pregnancy (35.6% vs. 11.5%, p=0.023), and implantation (18.1% vs. 5.7%, p=0.007) compared with chemical ZD. Among non-transferred embryos, the rate of blastocyst formation on day 5 (49.1% vs. 39.5%, p=0.016) and hatching on day 6 (47.2% vs. 26.5%, p<0.001) were also significantly higher in the PZD group.
CONCLUSION
The mechanical zona dissection method showed better outcomes than chemical ZD in terms of the blastocyst development and pregnancy rate. In this study, the fact that chemical ZD was conducted in different period from mechanical method should be considered in interpreting the result.

Keyword

Embryo biopsy; Partial zona dissection; Zona drilling; Acid Tyrode's solution

MeSH Terms

Blastocyst
Embryonic Structures
Family Characteristics
Herpes Zoster
Isotonic Solutions
Mandrillus
Pregnancy
Pregnancy Rate
Preimplantation Diagnosis
Prostaglandins D
Zona Pellucida
Isotonic Solutions
Prostaglandins D