Korean J Nutr.  2010 Aug;43(4):357-366. 10.4163/kjn.2010.43.4.357.

Genetic Variants Associated with Calorie and Macronutrient Intake in a Genome-Wide Association Study

• Baik I
• Ahn Y
• Lee SK
• Kim S
• Han BG
• Shin C

• KMID: 2268024
• DOI: http://doi.org/10.4163/kjn.2010.43.4.357

Abstract

There has been no genome-wide association study (GWAS) for macronutrient intake as a quantitative trait. To explore genetic loci associated with total calorie and macronutrient intake, genome-wide association data of autosomal single nucleotide polymorphisms (SNPs) from Korean adults were analyzed. We conducted a GWAS in 3,690 men and women aged 40 to 60 years from an urban population-based cohort. At the baseline examination (June 18, 2001 through January 29, 2003), DNA samples of the study subjects were collected and analyzed for genotyping. The information of average daily consumption of total calorie, carbohydrate, protein, and fat was obtained from a semi-quantitative food frequency questionnaire and transformed by natural logarithm for analyses after adjustment of calorie intake. Using multivariate linear regression analysis adjusted for age, sex, and height, we tested for 352,021 SNPs and found weak associations, which do not reach genome-wide association significance, with calorie and macronutrient intake. However, a number of SNPs were found to have potential associations with macronutrient intake; in particular, signals in SORBS1 and those in PRKCB1 were likely associated with carbohydrate and fat intake, respectively. We observed an inverse association between the minor allele of the SNPs in these genes and the amount of consumption of carbohydrate or fat. Our GWAS identified loci and minor alleles weakly associated with macronutrient intake. Because SORBS1 and PRKCB1 are reportedly associated with the metabolism of glucose and lipid as well as with obesity-related diseases, further investigations on biological and functional roles of polymorphism of these genes in the relation to macronutrient intake are warranted.

Keyword

calorie; macronutrient; single nucleotide polymorphisms; genome-wide association study

MeSH Terms

Adult
Aged
Alleles
Cohort Studies
DNA
Female
Genetic Loci
Genome-Wide Association Study
Glucose
Humans
Linear Models
Lipid A
Male
Polymorphism, Single Nucleotide
Surveys and Questionnaires
DNA
Glucose
Lipid A

Figure

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  Fig. 1 Quantile-quantile (QQ) plots of the -log10 p-values from the additive model-based analysis of genome-wide association data for average daily intake of total calorie and macronutrients among 3,690 study subjects. Amounts of carbohydrate, protein, and fat consumption have been adjusted for total calorie and log-transformed.

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  Fig. 2 Association analysis of SNPs in SORBS1 gene region on chromosome 10q23.33. The panel shows p-values for the association testing of genome-wide association data with natural log transformed calorie-adjusted carbohydrate intake. The association was drawn from linear regression analysis adjusted for sex, age, and height on the basis of additive model. The Y axis is the negative log10 p-values and the × axis is position of chromosome 10q23.

• 

  Fig. 3 Association analysis of SNPs in PRKCB1 gene region on chromosome 16p12.1. The panel shows p-values for the association testing of genome-wide association data with natural log transformed calorie-adjusted fat intake. The association was drawn from linear regression analysis adjusted for sex, age, and height on the basis of dominant model. The Y axis is the negative log10 p-values and the × axis is position of chromosome 16p12.

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