Screening of the Differentially Expressed Proteins in Human Gynecological Tumors

Kim, N K; Chee, Y H; Lee, S H; Cha, K Y

Abstract

We compared with the differences of protein expression between normal and tumor tissues in 1 case of endometrial cancer, 2 cases of cervical cancer, 2 cases of benign ovarian tumor and 4 cases of ovarian malignancies including two borderline tumors and one metastatic tumor by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The results were as follows: 1. In one endometrial cancer, 12 kDa, 25 kDa, 31.5 kDa, 51.5 kDa, 62 kDa and 72 kDa proteins were overexpressed or up-regulated (lane 2, 3), and a 34.5 kDa protein was newly expressed (lane 3). 2. In 2 cervical cancers, 12 kDa, 25 kDa, 31.5 kDa, 51.5 kDa, 62 kDa and 72 kDa proteins were overexpressed or up-regulated, and a 40 kDa protein was down-regulated (lane 4, 5 and 6, 7). 43.5 kDa and 62 kDa proteins were overexpressed in one patient (lane 6, 7). 3. In 2 benign ovarian tumors, 18.8 kDa and 40 kDa proteins were down-regulated (lane 8, 9), and almost all bands were down-regulated except 14 kDa and 67 kDa proteins (lane 12). 4. In one ovarian cancer, 18.8 kDa, 24 kDa and 38 kDa (lane 10, 11) proteins were up-regulated. 5. In 2 borderline ovarian tumors, a 31.5 kDa (lane 13, lane 14) protein was down-regulated. 24 kDa (lane 13) and 42 kDa (lane 14) proteins were up-regulated, respectively. 6. In one metastatic ovarian cancer (lane 15), 42 kDa and 72 kDa proteins were up-regulated. The differential display in our experiment showed at least one new (34.5 kDa), 11 up-regulated (12, 24,25, 31.5, 38, 42, 43.5, 51.5, 62, 67, 72 kDa) and 2 down-regulated (18.8, 40 kDa) bands. The protein patterns were similar between endometrial and cervical cancer, except for a 34.5 kDa in endometrial and a 43.5 kDa in cervical cancer.