Korean J Blood Transfus.
2006 Dec;17(2):153-158.
A Patient with RhD(el) (1227G>A) Failed to Produce Detectable Anti-D after Transfusion of RhD Positive Red Blood Cells
- Affiliations
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- 1Department of Laboratory Medicine, Chonnam National University Medical School, Korea. hasomii@hanmail.net
- 2Gwangju-Jeonnam Red Cross Blood Center, Korea.
- 3Department of Neurology, Chonnam National University Medical School, Gwangju, Korea.
Abstract
- Only 0.15% of all donors in Korea are RhD negative, which has led to a chronic shortage of RhD negative blood. Most physicians are aware of the potential for RhD alloimmunization after transfusing RhD+ red blood cells into RhD- patients. Hence, the undertransfusion of RhD- patients might be occurring in Korea. A 66-year-old man without a history of transfusion tested negative for anti-D in his serum. In an emergency situation where RhD- blood was unavailable, the patient received two units of RhD+ RBCs. Anti-D was not detected over three months after the transfusion. The red cells of the patient showed no agglutination with the anti-D reagent and a negative result by the standard weak D test. The polymerase chain reaction-sequence specific primers (PCR-SSP) and sequencing revealed D(el) (1227G>A) in the patient.